Anti-PYK2 (phospho Y881) antibody (ab4801)
Key features and details
- Rabbit polyclonal to PYK2 (phospho Y881)
- Suitable for: WB
- Reacts with: Chicken
- Isotype: IgG
Overview
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Product name
Anti-PYK2 (phospho Y881) antibody
See all PYK2 primary antibodies -
Description
Rabbit polyclonal to PYK2 (phospho Y881) -
Host species
Rabbit -
Specificity
Some cross-reactivity with Focal Adhesion Kinase phosphorylated at tyrosine 925 (the corresponding Grb 2 binding site) may be observed in cases where Focal Adhesion Kinase is overexpressed relative to PYK 2 in the same cell system. -
Tested Applications & Species
See all applications and species dataApplication Species WB Chicken
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Immunogen
Synthetic peptide (Human) derived from the region of human Pyk 2 that contains tyrosine 881.
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Positive control
- WB: Chicken embryo fibroblasts plated on fibronectin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit serum by epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PYK 2. The final product is generated by affinity chromatography using a PYK 2-derived peptide that is phosphorylated at tyrosine 881. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PYK2 (phospho Y881) antibody (ab4801)All lanes : Anti-PYK2 (phospho Y881) antibody (ab4801) at 1/1000 dilution
Lane 1 : Mock-transfected - Primary chicken embryo fibroblasts plated on fibronectin, pervanadate-treated
Lane 2 : Only expression vector transfected - Primary chicken embryo fibroblasts plated on fibronectin, pervanadate-treated
Lane 3 : Pyk2 (pY^881)NP expression vector transfected - Primary chicken embryo fibroblasts plated on fibronectin, pervanadate-treated
Lane 4 : FAK (pY^925) expression vector transfected- Primary chicken embryo fibroblasts plated on fibronectin, pervanadate-treated
Lane 5 : Generic pY expression vector transfected - Primary chicken embryo fibroblasts plated on fibronectin, pervanadate-treated
Lane 6 : Pyk2 (pY^881)P - Primary chicken embryo fibroblasts plated on fibronectin, pervanadate-treated
Predicted band size: 116 kDa
