Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [YE353] to PYK2 - BSA and Azide free
  • Suitable for: WB, ICC/IF, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PYK2 antibody [YE353] - BSA and Azide free
    See all PYK2 primary antibodies

  • Description

    Rabbit monoclonal [YE353] to PYK2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Ramos, Jurkat and RAW264.7 cell lysates and mouse and rat brain tissue lysates. IHC-P: Human, mouse and rat cerebral cortex tissues. ICC/IF: HeLa and PC12 cells.

  • General notes

    ab228477 is the carrier-free version of ab32571.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Western blot - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)
    Western blot - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)

    This WB data was generated using the same anti-PYK2 antibody clone, YE353, in a different buffer formulation (cat# ab32571).

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)              
    Lane 2: PYK2 knockout  HAP1 whole cell lysate (20 µg)
    Lane 3: Jurkat whole cell lysate (20 µg)
    Lane 4: Hu brain whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab32571 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab32571 was shown to recognize PYK2 when PYK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and PYK2 knockout samples were subjected to SDS-PAGE.  Ab32571 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)
    Immunocytochemistry/ Immunofluorescence - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PYK2 with purified ab32571 at 1/60. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32571).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebral cortex tissue labelling PYK2 with purified ab32571 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32571).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebral cortex tissue labelling PYK2 with purified ab32571 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32571).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling PYK2 with purified ab32571 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32571).

  • Immunocytochemistry/ Immunofluorescence - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)
    Immunocytochemistry/ Immunofluorescence - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)

    Immunocytochemistry/Immunofluorescence analysis of PC12 cells labelling PYK2 with unpurified ab32571 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32571).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)

    This IHC data was generated using the same anti-PYK2 antibody clone, YE353, in a different buffer formulation (cat# ab32571).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human brain tissue labelling PYK2 with unpurified ab32571 at a 1/250 dilution.

  • Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)
    Anti-PYK2 antibody [YE353] - BSA and Azide free (ab228477)

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