Anti-PYK2 (phospho Y579) antibody (ab4805)
Key features and details
- Rabbit polyclonal to PYK2 (phospho Y579)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PYK2 (phospho Y579) antibody
See all PYK2 primary antibodies -
Description
Rabbit polyclonal to PYK2 (phospho Y579) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide (Human) derived from the region of human Pyk 2 that contains tyrosine 579. The sequence is conserved in mouse and rat.
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Positive control
- Chicken embryo fibroblasts expressing human PYK 2 protein and plated on fibronectin.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PYK 2, (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence, and (iii) a phosphopeptide derived from the corresponding region of Focal Adhesion Kinase (a PYK 2-related protein) to remove antibody that is reactive with the phosphorylated Focal Adhesion Kinase protein. The final product is generated by affinity chromatography using a PYK 2-derived peptide that is phosphorylated at tyrosine 579. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Peptide Competition: Extracts prepared from chick embryo fibroblasts expressing Pyk2 and plated on fibronectin were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to nitrocellulose. Membranes were blocked with a 4% BSA-TBST buffer overnight at 4oC, then were incubated with 0.25
µ g/mL ab4805 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the phosphopeptide immunogen (2), the nonphosphopeptide corresponding to the immunogen (3), a phosphopeptide derived from the corresponding region of FAK (4) or, a generic phosphotyrosine containing peptide (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab4805 comepletely blocks the antibody signal, thereby demonstrating the specificity of the antibody. Peptide Compe