Anti-PYK2 antibody [E354] (ab32448)
![Anti-PYK2 antibody [E354] (ab32448)](https://www.abcam.com/ps/products/32/ab32448/Images/ab32448-281782-anti-pyk2-antibody-e354-western-blot.jpg "Anti-PYK2 antibody [E354] (ab32448)")
Key features and details
- Rabbit monoclonal [E354] to PYK2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-PYK2 antibody [E354]
See all PYK2 primary antibodies -
Description
Rabbit monoclonal [E354] to PYK2 -
Host species
Rabbit -
Specificity
The antibody does not cross-react with other Fak family members. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide within Rat PYK2 aa 950-1050 (C terminal). The exact sequence is proprietary.
-
Positive control
- Jurkat whole cell lysate (ab7899).
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
E354 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-PYK2 antibody [E354] (ab32448)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: PYK2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Jurkat whole cell lysate (20 µg)
Lane 4: Hu brain whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab32448 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32448 was shown to specifically react with PYK2 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when PYK2 knockout samples were used. Wild-type and PYK2 knockout samples were subjected to SDS-PAGE. Ab32448 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-PYK2 antibody [E354] (ab32448)](https://www.abcam.com/ps/products/32/ab32448/Images/ab32448-44886-anti-pyk2-antibody-e354-western-blot.gif)
Western blot - Anti-PYK2 antibody [E354] (ab32448)Anti-PYK2 antibody [E354] (ab32448) at 1/500 dilution + Jurkat cell lysate
Predicted band size: 116 kDa
Observed band size: 116 kDa
