Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspase-9 antibody [E84] (ab32068)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E84] to Caspase-9
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Caspase-9 antibody [E84]
    See all Caspase-9 primary antibodies

  • Description

    Rabbit monoclonal [E84] to Caspase-9

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Caspase-9 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • Jurkat cell lysate

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Caspase-9 antibody [E84] (ab32068)
    Western blot - Anti-Caspase-9 antibody [E84] (ab32068)

    Lanes:
    Lane 1: Wild-type HAP1 whole cell lysate (40 µg).
    Lane 2: CASP9 knockout HAP1 whole cell lysate (40µg).
    Lane 3: HeLa whole cell lysate (40 µg).

    Lanes 1 - 3 Merged signal (red and green). Green - ab32068 observed at 45 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab32068 was shown to specifically recognize CASP9 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when CASP9 knockout samples were examined. Wild-type and CASP9 knockout samples were subjected to SDS-PAGE. Ab32068 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Caspase-9 antibody [E84] (ab32068)
    Western blot - Anti-Caspase-9 antibody [E84] (ab32068)
    All lanes : Anti-Caspase-9 antibody [E84] (ab32068) at 1/1000 dilution

    Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa treated with staurosporine 1uM for 4 hours whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 46 kDa
    Additional bands at: 35 kDa, 37 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody [E84] (ab32068)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody [E84] (ab32068)

    Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-Pro-Caspase-9 RabMAb (ab32068).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Caspase-9 antibody [E84] (ab32068)
    Western blot - Anti-Caspase-9 antibody [E84] (ab32068)
    Anti-Caspase-9 antibody [E84] (ab32068) at 1/2000 dilution + Jurkat cell lysate at 10 µg

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

  • Anti-Caspase-9 antibody [E84] (ab32068)
    Anti-Caspase-9 antibody [E84] (ab32068)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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