Anti-PYK2 antibody (ab226798)
Key features and details
- Rabbit polyclonal to PYK2
- Suitable for: IP, IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PYK2 antibody
See all PYK2 primary antibodies -
Description
Rabbit polyclonal to PYK2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment within Human PYK2 (internal sequence). The exact sequence is proprietary.
Database link: Q14289 -
Positive control
- WB: A431 and HeLa whole cell extracts; Mouse and rat brain extracts. IP: A431 whole cell extract. ICC/IF: HeLa cells. IHC-P: Rat brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PYK2 antibody (ab226798)All lanes : Anti-PYK2 antibody (ab226798) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 116 kDa7.5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-PYK2 antibody (ab226798)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PYK2 (green) using ab226798 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Blue: Hoechst 33342 staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 antibody (ab226798)Paraffin-embedded rat brain tissue stained for PYK2 using ab226798 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-PYK2 antibody (ab226798)All lanes : Anti-PYK2 antibody (ab226798) at 1/1000 dilution
Lane 1 : Mouse brain extract
Lane 2 : Rat brain extract
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 116 kDa7.5% SDS-PAGE gel.
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Immunoprecipitation - Anti-PYK2 antibody (ab226798)PYK2 was immunoprecipitated from A431 (human epidermoid carcinoma cell line) whole cell extract with 5 µg ab226798. Western blot was performed from the immunoprecipitate using ab226798.
Lane 1: A431 whole cell extract.
Lane 1: Control IgG IP in A431 whole cell extract.
Lane 2: ab226798 IP in A431 whole cell extract.
Anti-Rabbit IgG was used as a secondary reagent.
