Anti-Src (phospho Y418) antibody (ab4816)
Key features and details
- Rabbit polyclonal to Src (phospho Y418)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Src (phospho Y418) antibody
See all Src primary antibodies -
Description
Rabbit polyclonal to Src (phospho Y418) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide derived from the region of Src that contains tyrosine 418 (tyrosine 419 including the initiating methionine). The sequence is conserved in human, mouse, rat, chicken and frog.
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General notes
Src (also known as pp60src) is a non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. Tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418 (tyrosine 414 on frog Src, tyrosine 416 on chicken Src, and tyrosine 424 on mouse Src). This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively pre-adsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Src, and (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a Src-derived peptide that is phosphorylated at tyrosine 418. -
Primary antibody notes
Src (also known as pp60src) is a non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. Tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418 (tyrosine 414 on frog Src, tyrosine 416 on chicken Src, and tyrosine 424 on mouse Src). This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src (phospho Y418) antibody (ab4816)
Immunohistochemical analysis of paraffin embedded Human colon carcinoma, labelling Src with ab4816. A negative control without ab4816 is shown on the left. Antigen retrieval performed using 10mM sodium citrate, microwaved for 8-15mins, blocked using 3% H2O2-methanol, then treated with ab4816 followed by an HRP-conjugated secondary antibody. Counterstaining with hematoxylin.
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Western blot - Anti-Src (phospho Y418) antibody (ab4816) Dr. Michael Schaller, Dept. of Cell Bio. & Anat., Univ. of North Carolina, Chapel Hill, NC.Western blot detection of Src phosphorylation in extracts of chick embryo fibroblasts expressing wild-type (lanes 2,4,6) or mutant (lanes 1,3,5) pp60src. Truncation of Src at position 518 eliminated phosphorylation at the negative regulatory site [pY529], while increasing phosphorylation at the catalytic site [pY418].
Western blot detection of Src phosphorylation in extracts of chick embryo fibroblasts expressing wild-type (lanes 2,4,6) or mutant (lanes 1,3,5) pp60src. Truncation of Src at position 518 eliminated phosphorylation at the negative regulatory site [pY529], while increasing phosphorylation at the catalytic site [pY418].
