Anti-Src (phospho Y418) antibody [EPR6239(2)] (ab133460)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6239(2)] to Src (phospho Y418)
- Suitable for: WB
- Reacts with: Rat, Human
Overview
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Product name
Anti-Src (phospho Y418) antibody [EPR6239(2)]
See all Src primary antibodies -
Description
Rabbit monoclonal [EPR6239(2)] to Src (phospho Y418) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB RatHuman -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Src (phospho Y418).
Database link: P12931 -
Positive control
- A431 cell lysate treated with Pervanadate
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6239(2) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Src (phospho Y418) antibody [EPR6239(2)] (ab133460) at 1/1000 dilution
Lane 1 : Untreated C6 (rat glioma) whole cell lysate
Lane 2 : C6 (rat glioma) treated with pervanadate whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 60 kDaPurified format.
Blocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM/TBST
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All lanes : Anti-Src (phospho Y418) antibody [EPR6239(2)] (ab133460) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma) treated with pervanadate whole cell lysate
Lane 2 : Untreated HeLa (human cervix adenocarcinoma) treated with pervanadate whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 60 kDaPurified format.
Blocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM/TBST
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All lanes : Anti-Src (phospho Y418) antibody [EPR6239(2)] (ab133460) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma) treated with pervanadate whole cell lysate
Lane 2 : Untreated A431 (human epidermoid carcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 60 kDaPurified format.
Blocking Buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM /TBST
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All lanes : Anti-Src (phospho Y418) antibody [EPR6239(2)] (ab133460) at 1/1000 dilution (undiluted)
Lane 1 : A431 cell lysate untreated
Lane 2 : A431 cell lysate treated with Pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 60 kDa
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Undiluted format.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD -