Antibodies, Proteins, Kits and Reagents for Life Science

526 012 ₸ Product size

100 µg 526 012 ₸ 1 mg 4 690 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Src antibody [EGTR103] - BSA and Azide free (ab222221)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EGTR103] to Src - BSA and Azide free
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-Src antibody [EGTR103] - BSA and Azide free
See all Src primary antibodies
Description

Rabbit monoclonal [EGTR103] to Src - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Rat and Mouse colon tissues. Human liver tissue. WB: HAP1, A431, U87-MG, and SH-5YSY cell lysates. Mouse and rat brain lysate. Mouse hippocampus lysate
General notes
Ab222221 is the carrier-free version of ab133283. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab222221 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EGTR103
Isotype

IgG
Research areas

Western blot - Anti-Src antibody [EGTR103] - BSA and Azide free (ab222221)

Anti-Src antibody [EGTR103] (ab133283) at 1/2000 dilution + U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 60 kDa
Observed band size: 60 kDa

This data was developed using ab133283, the same antibody clone in a different buffer formulation.
Western blot - Anti-Src antibody [EGTR103] - BSA and Azide free (ab222221)

All lanes : Anti-Src antibody [EGTR103] (ab133283) at 1/1000 dilution (Purified)

Lane 1 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 20 µg
Lane 2 : Mouse brain lysate at 20 µg
Lane 3 : Rat brain lysate at 20 µg/ml
Lane 4 : Mouse hippocampus lysate at 20 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 60 kDa
Observed band size: 60 kDa

This data was developed using ab133283, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src antibody [EGTR103] - BSA and Azide free (ab222221)

This data was developed using ab133283, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue sections labeling Src with purified ab133283 at 1/50 dilution (6.98 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src antibody [EGTR103] - BSA and Azide free (ab222221)

This data was developed using ab133283, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue sections labeling Src with purified ab133283 at 1/50 dilution (6.98 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src antibody [EGTR103] - BSA and Azide free (ab222221)

This data was developed using ab133283, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Src with purified ab133283 at 1/50 dilution (6.98 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-Src antibody [EGTR103] - BSA and Azide free (ab222221)

This data was developed using ab133283, the same antibody clone in a different buffer formulation.

Lane 1 Wild-type HAP1 cell lysate (20 µg)

Lane 2 Src knockout HAP1 cell lysate (20 µg)

Lane 3 HeLa cell lysate (20 µg)

Lane 4 A431 knockout HAP1 cell lysate (20 µg)

Lanes 1 - 4 Merged signal (red and green). Green - ab133283 observed at 60 kDa. Red - loading control, ab8226, observed at 42 kDa.

Unpurified ab133283 was shown to specifically react with Src when Src knockout samples were used. Wild-type and Src knockout samples were subjected to SDS-PAGE. ab133283 and ab8226 (loading control to beta actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW)preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-Src antibody [EGTR103] - BSA and Azide free (ab222221)

All lanes : Anti-Src antibody [EGTR103] (ab133283) at 1/1000 dilution (Unpurified)

Lane 1 : U87-MG cell lysate
Lane 2 : SH-5YSY cell lysate

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 60 kDa

This data was developed using ab133283, the same antibody clone in a different buffer formulation.
Anti-Src antibody [EGTR103] - BSA and Azide free (ab222221)

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