Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Src antibody [EPR5496] (ab109381)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5496] to Src
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Src antibody [EPR5496]
    See all Src primary antibodies

  • Description

    Rabbit monoclonal [EPR5496] to Src

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Hela, 293T, SH-SY5Y and HUVEC cell lysates. IHC-P: Human kidney and colonic adenocarcinoma tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Src antibody [EPR5496] (ab109381)
    Western blot - Anti-Src antibody [EPR5496] (ab109381)

    Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
    Lanes 2, 6 and 10: Src knockout HAP1 cell lysate (20 µg)
    Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
    Lanes 4, 8 and 12: A431 cell lysate (20 µg)
    Lanes 1, 2, 3 and 4: Green signal from target – ab109381 observed at 60 kDa
    Lanes 5, 6, 7 and 8: Red signal from loading control – ab8245 observed at 37 kDa
    Lanes 9, 10, 11 and 12: Merged (red and green) signal

    ab109381 was shown to specifically react with Src when Src knockout samples were used. Wild-type and Src knockout samples were subjected to SDS-PAGE. ab109381 and ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-Src antibody [EPR5496] (ab109381)
    Western blot - Anti-Src antibody [EPR5496] (ab109381)
    All lanes : Anti-Src antibody [EPR5496] (ab109381) at 1/10000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : SH-SY5Y cell lysate
    Lane 4 : HUVEC cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 60 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src antibody [EPR5496] (ab109381)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src antibody [EPR5496] (ab109381)

    ab109381, at 1/250 dilution, staining Src in paraffin-embedded Human kidney tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Src antibody [EPR5496] (ab109381)
    Western blot - Anti-Src antibody [EPR5496] (ab109381)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Src knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)

     

    Lanes 1 - 4: Merged signal (red and green). Green - ab109381 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.

    This western blot image is a comparison between ab109381 and a competitor's top cited rabbit polyclonal antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src antibody [EPR5496] (ab109381)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src antibody [EPR5496] (ab109381)

    ab109381, at 1/250 dilution, staining Src in paraffin-embedded Human colonic adenocarcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Src antibody [EPR5496] (ab109381)
    Anti-Src antibody [EPR5496] (ab109381)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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