Anti-Src antibody [EPR5496] (ab109381)
![Anti-Src antibody [EPR5496] (ab109381)](https://www.abcam.com/ps/products/109/ab109381/Images/ab109381-251975-anti-src-antibody-epr5496-western-blot.jpg "Anti-Src antibody [EPR5496] (ab109381)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5496] to Src
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Src antibody [EPR5496]
See all Src primary antibodies -
Description
Rabbit monoclonal [EPR5496] to Src -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Hela, 293T, SH-SY5Y and HUVEC cell lysates. IHC-P: Human kidney and colonic adenocarcinoma tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5496 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Src antibody [EPR5496] (ab109381)
Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 6 and 10: Src knockout HAP1 cell lysate (20 µg)
Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
Lanes 4, 8 and 12: A431 cell lysate (20 µg)
Lanes 1, 2, 3 and 4: Green signal from target – ab109381 observed at 60 kDa
Lanes 5, 6, 7 and 8: Red signal from loading control – ab8245 observed at 37 kDa
Lanes 9, 10, 11 and 12: Merged (red and green) signalab109381 was shown to specifically react with Src when Src knockout samples were used. Wild-type and Src knockout samples were subjected to SDS-PAGE. ab109381 and ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-Src antibody [EPR5496] (ab109381)](https://www.abcam.com/ps/products/109/ab109381/Images/ab109381-122167-anti-src-antibody-epr5496-western-blot.jpg)
Western blot - Anti-Src antibody [EPR5496] (ab109381)All lanes : Anti-Src antibody [EPR5496] (ab109381) at 1/10000 dilution
Lane 1 : Hela cell lysate
Lane 2 : 293T cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 60 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src antibody [EPR5496] (ab109381)](https://www.abcam.com/ps/products/109/ab109381/Images/ab109381-122166-anti-src-antibody-epr5496-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src antibody [EPR5496] (ab109381)ab109381, at 1/250 dilution, staining Src in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Src antibody [EPR5496] (ab109381)](https://www.abcam.com/ps/products/109/ab109381/Images/ab109381-276789-anti-src-antibody-epr5496-western-blot.jpg)
Western blot - Anti-Src antibody [EPR5496] (ab109381)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Src knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: A431 cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab109381 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.
This western blot image is a comparison between ab109381 and a competitor's top cited rabbit polyclonal antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src antibody [EPR5496] (ab109381)](https://www.abcam.com/ps/products/109/ab109381/Images/ab109381-122165-anti-src-antibody-epr5496-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src antibody [EPR5496] (ab109381)ab109381, at 1/250 dilution, staining Src in paraffin-embedded Human colonic adenocarcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Src antibody [EPR5496] (ab109381)](https://www.abcam.com/ps/products/109/ab109381/Images/ab109381-8-benefits-of-recombinant-antibodies.png)
Anti-Src antibody [EPR5496] (ab109381)
