Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Src antibody [GD11] - BSA and Azide free (ab269563)

Key features and details

  • Mouse monoclonal [GD11] to Src - BSA and Azide free
  • Suitable for: WB, ICC/IF, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Src antibody [GD11] - BSA and Azide free
    See all Src primary antibodies

  • Description

    Mouse monoclonal [GD11] to Src - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment corresponding to Chicken Src.

  • Epitope

    The GD11 monoclonal binds to amino acid residues 82 to 169 of the Src protein (PubMed ID: 6205164).

  • Positive control

    • WB: HAP1, A431, Mouse and rat testes tissue lysates. ICC/IF: HAP1 wildtype and HAP1-SRC knockout cells. IHC-P: FFPE human testis tissue sections.

  • General notes

    ab269563 is the carrier-free version of ab231081.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Western blot - Anti-Src antibody [GD11] - BSA and Azide free (ab269563)
    Western blot - Anti-Src antibody [GD11] - BSA and Azide free (ab269563)
    All lanes :

    Lane 1 : HAP1 whole cell lysate
    Lane 2 : HAP1 Src KO whole cell lysate
    Lane 3 : A431 whole cell lysate
    Lane 4 : Mouse testes whole tissue lysate
    Lane 5 : Rat testes whole tissue lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 60 kDa



    ab231081 was shown to specifically react with Src in wild type HAP1 cells. No band was observed when Src knockout samples were used. Wild-type and Src knockout samples were subjected to SDS-PAGE. ab231081 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1ug/ml concentration and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This image was produced using the same antibody clone but in a different formulation ab231081, PBS and sodium azide.

  • Immunocytochemistry/ Immunofluorescence - Anti-Src antibody [GD11] - BSA and Azide free (ab269563)
    Immunocytochemistry/ Immunofluorescence - Anti-Src antibody [GD11] - BSA and Azide free (ab269563)

    ab231081 staining Src in wild-type HAP1 cells (top panel) and SRC knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab231081 at 5μg/ml and ab6046 (Rabbit polyclonal to beta tubulin - loading control) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with ab150117 (Goat secondary antibody to Mouse IgG (Alexa Fluor® 488) at 2 μg/ml (colored green) and ab150084 (Goat secondary antibody to Rabbit IgG (Alexa Fluor® 594) at 2 μg/ml (pseudo color red). Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This image was produced using the same antibody clone but in a different formulation ab231081, PBS and sodium azide.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src antibody [GD11] - BSA and Azide free (ab269563)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src antibody [GD11] - BSA and Azide free (ab269563)

    IHC image of Src staining in a section of formalin-fixed paraffin-embedded human normal testis* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab231081, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    This image was produced using the same antibody clone but in a different formulation ab231081, PBS and sodium azide.

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