Anti-Lyn antibody (ab227170)
Key features and details
- Rabbit polyclonal to Lyn
- Suitable for: ICC/IF, IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Lyn antibody
See all Lyn primary antibodies -
Description
Rabbit polyclonal to Lyn -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Cow
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Immunogen
Recombinant fragment within Human Lyn (internal sequence). The exact sequence is proprietary.
Database link: P07948 -
Positive control
- WB: THP-1 whole cell lysate. IP: K562 whole cell extract. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Lyn antibody (ab227170)Anti-Lyn antibody (ab227170) at 1/1000 dilution + THP-1 (human monocytic leukemia cell line) whole cell lysate at 30 µg
Predicted band size: 59 kDa7.5 % SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-Lyn antibody (ab227170)PFA-fixed A431 (human epidermoid carcinoma cell line) cells stained for Lyn (green) using ab227170 at 1/500 dilution in ICC/IF.
Counterstain: Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (red) at 1/2000 dilution.
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Immunoprecipitation - Anti-Lyn antibody (ab227170)Lyn was immunoprecipitated from K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell extract with 4 µg of ab227170. Western blot was performed from the immunoprecipitate using ab227170 at 1/500 dilution. An Anti-Rabbit IgG was used as secondary antibody.
Lane 1: K562 whole cell lysate 40 µg (Input).
Lane 2: Rabbit IgG instead of ab227170 in K562 whole cell lysate.
Lane 3: ab227170 IP in K562 whole cell lysate.
