Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914)

Key features and details

  • Rabbit monoclonal [EP504Y] to Lyn (phospho Y507)
  • Suitable for: Dot blot, WB, IP
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Lyn (phospho Y507) antibody [EP504Y]
    See all Lyn primary antibodies

  • Description

    Rabbit monoclonal [EP504Y] to Lyn (phospho Y507)

  • Host species

    Rabbit

  • Specificity

    The antibody will detect Lyn’s phosphorylation on Tyrosine 507.

  • Tested applications

    Suitable for: Dot blot, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human Lyn (phospho Y507). The exact sequence is proprietary.
    Database link: P07948

  • Positive control

    • WB: HeLa (untreated and treated with pervanadate) and Raji treated with pervanadate whole cell lysates. IP: SH-SY5Y treated with pervandate whole cell lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP504Y

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914)
    Western blot - Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914)
    All lanes : Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914) at 1/1000 dilution

    Lane 1 : Raji whole cell lysate
    Lane 2 : Raji treated with 1mM pervanadate for 30 min whole cell lysate
    Lane 3 : Raji treated with 1mM pervanadate for 30 min whole cell lysate, the membrane was incubated with alkaline phosphatase.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 61 kDa
    Observed band size: 61 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914)
    Immunoprecipitation - Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914)

    Purified ab33914 at 1/80 dilution (2µg) immunoprecipitating Lyn in SH-SY5Y treated with pervandate whole cell lysate.
    Lane 1 (input): SH-SY5Y (Human neuroblastoma epithelial cell) treated with pervandate whole cell lysate 10µg
    Lane 2 (+): ab33914 + SH-SY5Y treated with pervandate whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab256224 in SH-SY5Y treated with pervandate whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 61 kDa

  • Dot Blot - Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914)
    Dot Blot - Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914)

    Dot blot analysis of Lyn (phospho Y507) phospho peptide (Lane 1) and Lyn non-phospho peptide (Lane 2) labelling Lyn (phospho Y507) with ab33914 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/100,000.

    Blocking and dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914)
    Western blot - Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914)
    All lanes : Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914) at 1/5000 dilution

    Lane 1 : 10ug Non treated HeLa cell lysate
    Lane 2 : 10ug HeLa cell lysate treated with Pervanadate

    Secondary
    All lanes : Goat anti-rabbit HRP labeled.

    Predicted band size: 61 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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