PE Anti-SRC Family (phospho Y418) antibody [EP503Y] (ab208828)
![PE Anti-SRC Family (phospho Y418) antibody [EP503Y] (ab208828)](https://www.abcam.com/ps/products/208/ab208828/Images/ab208828-268651-ICCAP00253831.jpg "PE Anti-SRC Family (phospho Y418) antibody [EP503Y] (ab208828)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EP503Y] to SRC Family (phospho Y418)
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-SRC Family (phospho Y418) antibody [EP503Y]
See all SRC Family primary antibodies -
Description
PE Rabbit monoclonal [EP503Y] to SRC Family (phospho Y418) -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
ab208828 recognises Lyn phosphorylation on Tyrosine 396. BLAST analysis show 100% homology with Hck (pY410) and Lck (pY393), and 92% homology with Src (pY418), Fyn (pY419) and Yes (pY425) Therefore, the antibody may cross-react with these proteins. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: pervanadate-treated HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP503Y -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - PE Anti-SRC Family (phospho Y418) antibody [EP503Y] (ab208828)
ab208828 staining SRC Family (phospho Y418) in HeLa cells +/- pervanadate (1mM, 15min), then treated with LP (31 degrees C, 2 hours). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h.
The cells were then incubated overnight at +4°C with ab208828 at 1/100 dilution (pseudocolored in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![PE Anti-SRC Family (phospho Y418) antibody [EP503Y] (ab208828)](https://www.abcam.com/ps/products/208/ab208828/Images/ab208828-2-benefits-of-recombinant-antibodies.png)
PE Anti-SRC Family (phospho Y418) antibody [EP503Y] (ab208828)
