Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Lyn antibody [Y497] (ab32398)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y497] to Lyn
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Lyn antibody [Y497]
    See all Lyn primary antibodies

  • Description

    Rabbit monoclonal [Y497] to Lyn

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Lyn aa 1-100. The exact sequence is proprietary.
    Database link: P07948

  • Epitope

    Residues close to SH3 domain of human Lyn

  • Positive control

    • WB: HAP1, Ramos, PC-12, and A431 whole cell lysates. Mouse lung, and mouse and rat liver tissue lysates.

  • General notes

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    Y497

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Lyn antibody [Y497] (ab32398)
    Western blot - Anti-Lyn antibody [Y497] (ab32398)
    All lanes : Anti-Lyn antibody [Y497] (ab32398) at 1/1000 dilution (Purified)

    Lane 1 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
    Lane 2 : Mouse lung lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 4 : Rat liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 53 kDa
    Observed band size: 56 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

  • Western blot - Anti-Lyn antibody [Y497] (ab32398)
    Western blot - Anti-Lyn antibody [Y497] (ab32398)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Lyn knockout HAP1 cell lysate (20 µg)
    Lane 3: Ramos cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab32398 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab32398 was shown to specifically react with Lyn when Lyn knockout samples were used. Wild-type and Lyn knockout samples were subjected to SDS-PAGE. ab32398 and ab8245 (loading control to GAPDH) were diluted at 1/2000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-Lyn antibody [Y497] (ab32398)
    Western blot - Anti-Lyn antibody [Y497] (ab32398)
    All lanes : Anti-Lyn antibody [Y497] (ab32398) at 1/2000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?



    Lanes 1 - 3: Merged signal (red and green). Green - ab32398 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa. 

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab32398 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at a 1:10000 dilution for 1hr at room temperature and then imaged.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Lyn antibody [Y497] (ab32398)

© 2021 Astana Biomed Group LLP. All rights reserved.