Lane 1: Wild-type HeLa cell lysate (20µg)

Lane 2: YES1 knockout HeLa cell lysate (20µg)
Lane 3: SW480 cell lysate (20µg)
Lane 4: Daudi cell lysate (20µg)
Lanes 1- 4: Merged signal (red and green). Green - ab109265 observed at 60 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab109265 Anti-Yes1 antibody [EPR3173] was shown to specifically react with Yes1 in wild-type HeLa cells in western blot. The band observed in the knockout cell line ab265202 (knockout cell lysate ab258290) lane below 60kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and Yes1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109265 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

Allele-1: 31 bp deletion in exon2

 

Allele-2: 14 bp deletion in exon2

 

Allele-3: 8 bp deletion in exon2