Anti-Lyn (phospho Y507) antibody [EP504Y] - BSA and Azide free (ab256224)
![Anti-Lyn (phospho Y507) antibody [EP504Y] - BSA and Azide free (ab256224)](https://www.abcam.com/ps/products/256/ab256224/Images/ab256224-349841-antilyn-phospho-y507-antibody-ep504y-western-blot-raji-human.jpg "Anti-Lyn (phospho Y507) antibody [EP504Y] - BSA and Azide free (ab256224)")
Key features and details
- Rabbit monoclonal [EP504Y] to Lyn (phospho Y507) - BSA and Azide free
- Suitable for: WB, IP, Dot blot
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Lyn (phospho Y507) antibody [EP504Y] - BSA and Azide free
See all Lyn primary antibodies -
Description
Rabbit monoclonal [EP504Y] to Lyn (phospho Y507) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Dot blotmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa (untreated and treated with pervanadate) and Raji treated with pervanadate whole cell lysates. IP: SH-SY5Y treated with pervandate whole cell lysate.
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General notes
Ab256224 is the carrier-free version of ab33914. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab256224 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP504Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Lyn (phospho Y507) antibody [EP504Y] - BSA and Azide free (ab256224)All lanes : Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914) at 1/1000 dilution
Lane 1 : Raji whole cell lysate
Lane 2 : Raji treated with 1mM pervanadate for 30 min whole cell lysate
Lane 3 : Raji treated with 1mM pervanadate for 30 min whole cell lysate, the membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDaBlocking and dilution buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab33914).
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![Immunoprecipitation - Anti-Lyn (phospho Y507) antibody [EP504Y] - BSA and Azide free (ab256224)](https://www.abcam.com/ps/products/256/ab256224/Images/ab256224-2-anti-lyn-phospho-y507-antibody-ep504y-immunoprecipitation-shsy5y-human.jpg)
Immunoprecipitation - Anti-Lyn (phospho Y507) antibody [EP504Y] - BSA and Azide free (ab256224)Purified ab33914 at 1/80 dilution (2µg) immunoprecipitating Lyn in SH-SY5Y treated with pervandate whole cell lysate.
Lane 1 (input): SH-SY5Y (Human neuroblastoma epithelial cell) treated with pervandate whole cell lysate 10µg
Lane 2 (+): ab33914 + SH-SY5Y treated with pervandate whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab256224 in SH-SY5Y treated with pervandate whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 61 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab33914).
