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Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] - BSA and Azide free (ab232472)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7106] to Retinoid X Receptor alpha/RXRA - BSA and Azide free
  • Suitable for: ICC/IF, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] - BSA and Azide free
    See all Retinoid X Receptor alpha/RXRA primary antibodies

  • Description

    Rabbit monoclonal [EPR7106] to Retinoid X Receptor alpha/RXRA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: MCF7 cells.

  • General notes

    ab232472 is the carrier-free version of ab125001 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab232472 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Retinoid X Receptor alpha

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Immunoprecipitation - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] - BSA and Azide free (ab232472)
    Immunoprecipitation - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] - BSA and Azide free (ab232472)

    ab125001 (purified) at 1:20 dilution (0.6μg) immunoprecipitating Retinoid X Receptor alpha/RXRA in HeLa whole cell lysate.

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, 10μg. 
    Lane 2 (+): ab125001 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab125001 in HeLa whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab125001).

  • OI-RD Scanning - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] - BSA and Azide free (ab232472)
    OI-RD Scanning - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] - BSA and Azide free (ab232472)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab125001).

  • Immunocytochemistry/ Immunofluorescence - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] - BSA and Azide free (ab232472)
    Immunocytochemistry/ Immunofluorescence - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] - BSA and Azide free (ab232472)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling Retinoid X Receptor alpha/RXRA with Purified ab125001 at 1:500 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab125001).

  • Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] - BSA and Azide free (ab232472)
    Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] - BSA and Azide free (ab232472)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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