Anti-Retinoid X Receptor alpha/RXRA antibody (ab24363)
Key features and details
- Goat polyclonal to Retinoid X Receptor alpha/RXRA
- Suitable for: WB, Flow Cyt, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Retinoid X Receptor alpha/RXRA antibody
See all Retinoid X Receptor alpha/RXRA primary antibodies -
Description
Goat polyclonal to Retinoid X Receptor alpha/RXRA -
Host species
Goat -
Specificity
This does not cross-react with either RXR beta or gamma. -
Tested applications
Suitable for: WB, Flow Cyt, ICCmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human Retinoid X Receptor alpha/RXRA aa 14-28 (N terminal).
Sequence:(C)QVNSSLTSPTGRGSM
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General notes
This product was previously labelled as Retinoid X Receptor alpha
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Retinoid X Receptor alpha/RXRA antibody (ab24363)All lanes : Anti-Retinoid X Receptor alpha/RXRA antibody (ab24363) at 0.3 µg/ml
Lane 1 : HeLa nuclear lysate (in RIPA buffer)
Lane 2 : HeLa nuclear lysate (in RIPA buffer) with peptide
Lane 3 : K562 nuclear lysate (in RIPA buffer)
Lane 4 : K562 nuclear lysate (in RIPA buffer) with peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 51 kDa
Observed band size: 55-60 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected by chemiluminescence.
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Immunocytochemistry - Anti-Retinoid X Receptor alpha/RXRA antibody (ab24363)Immunocytochemistry analysis of MCF7 cells labelling Retinoid X Receptor alpha/RXRA with ab24363 at 10 µg/mL showing strong nuclear staining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. Primary incubation for 1 hour. Alexa Fluor® 488 secondary antibody at 2 µg/mL (green). Actin filaments were stained with phalloidin (red). Nuclear DNA was labelled with DAPI (blue).
Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
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Flow Cytometry - Anti-Retinoid X Receptor alpha/RXRA antibody (ab24363)Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) labelling Retinoid X Receptor alpha/RXRA with ab24363. Cells permeabilized with 0.5% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
