Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7106] to Retinoid X Receptor alpha/RXRA
  • Suitable for: WB, IP, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106]
    See all Retinoid X Receptor alpha/RXRA primary antibodies

  • Description

    Rabbit monoclonal [EPR7106] to Retinoid X Receptor alpha/RXRA

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Retinoid X Receptor alpha/RXRA (N terminal).

  • Positive control

    • WB: HCT116, MCF7, HeLa, K562, RAW 264.7, PC12 and NIH 3T3 cell lysates. ICC: MCF7 cells.

  • General notes

     This product was previously labelled as Retinoid X Receptor alpha

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    Western blot - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    All lanes : Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001) at 1/1000 dilution

    Lane 1 : Wild-type HCT116 cell lysate
    Lane 2 : RXRA knockout HCT116 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 51 kDa
    Observed band size: 53 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab125001 observed at 53 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab125001 was shown to react with Anti-Retinoid X Receptor alpha in HCT 116 wild-type cells in western blot with loss of signal observed in RXRA knockout cell line ab273708 (RXRA knockout cell lysate ab275245). HCT 116 wild-type and RXRA knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab125001 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    Western blot - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    All lanes : Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001) at 1/1000 dilution (purified)

    Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 51 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    Immunoprecipitation - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)

    ab125001 (purified) at 1:20 dilution (0.6μg) immunoprecipitating Retinoid X Receptor alpha/RXRA in HeLa whole cell lysate.

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, 10μg. 
    Lane 2 (+): ab125001 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab125001 in HeLa whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    Immunocytochemistry/ Immunofluorescence - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)

    Immunocytochemistry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Retinoid X Receptor alpha/RXRA with Purified ab125001 at 1:500 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    Western blot - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    All lanes : Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001) at 1/1000 dilution (unpurified)

    Lane 1 : MCF7 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : RAW 264.7 cell lysate
    Lane 5 : PC12 cell lysate
    Lane 6 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 51 kDa

  • OI-RD Scanning - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    OI-RD Scanning - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)
    Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab125001)

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