HRP Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab200782)
![HRP Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab200782)](https://www.abcam.com/ps/products/200/ab200782/Images/ab200782-248171-WBab2007821.jpg "HRP Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab200782)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR7106] to Retinoid X Receptor alpha/RXRA
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106]
See all Retinoid X Receptor alpha/RXRA primary antibodies -
Description
HRP Rabbit monoclonal [EPR7106] to Retinoid X Receptor alpha/RXRA -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MCF7, HeLa, K562, RAW 264.7, PC12 and NIH 3T3 whole cell lysates.
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General notes
This product was previously labelled as Retinoid X Receptor alpha
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Dissociation constant (KD)
KD = 1.40 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7106 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab200782)All lanes : HRP Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab200782) at 1/1000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 4 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 12 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab200782 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab200782)](https://www.abcam.com/ps/products/200/ab200782/Images/ab200782-3-benefits-of-recombinant-antibodies.png)
HRP Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (ab200782)
