Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cyclin A2 antibody [Y193] - BSA and Azide free (ab167392)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y193] to Cyclin A2 - BSA and Azide free
  • Suitable for: WB, IP, ICC/IF, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Cyclin A2 antibody [Y193] - BSA and Azide free
    See all Cyclin A2 primary antibodies

  • Description

    Rabbit monoclonal [Y193] to Cyclin A2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to Human Cyclin A2 (C terminal).

  • Positive control

    • WB: HeLa cell lysate.

  • General notes

    Ab167392 is the carrier-free version of ab32386. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab167392 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [Y193] - BSA and Azide free (ab167392)
    Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [Y193] - BSA and Azide free (ab167392)

    This ICC data was generated using the same anti-Cyclin A2 antibody clone, Y193, in a different buffer formulation (cat# ab32386).

    Immunofluorescence staining of MCF7 cells with purified ab32386 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4 % PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab32386 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Immunoprecipitation - Anti-Cyclin A2 antibody [Y193] - BSA and Azide free (ab167392)
    Immunoprecipitation - Anti-Cyclin A2 antibody [Y193] - BSA and Azide free (ab167392)

    ab32386 (purified) at 1/40 immunoprecipitating Cyclin A2 in 10 µg HeLa cell lysate (Lanes 1 and 2, observed at 55 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32386).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [Y193] - BSA and Azide free (ab167392)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [Y193] - BSA and Azide free (ab167392)

    This IHC data was generated using the same anti-Cyclin A2 antibody clone, Y193, in a different buffer formulation (cat# ab32386).

    Immunohistochemical staining of paraffin embedded human colon carcinoma with purified ab32386 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Anti-Cyclin A2 antibody [Y193] - BSA and Azide free (ab167392)
    Anti-Cyclin A2 antibody [Y193] - BSA and Azide free (ab167392)

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