Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Thursday March 04, 2021

Anti-Cyclin A2 antibody [E399] (ab32498)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [E399] to Cyclin A2
Suitable for: WB, ICC/IF, IP
Reacts with: Human

Product name

Anti-Cyclin A2 antibody [E399]
See all Cyclin A2 primary antibodies
Description

Rabbit monoclonal [E399] to Cyclin A2
Host species

Rabbit
Specificity

ab32498 recognises Cyclin A2.

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Cyclin A2 aa 100-200 (N terminal). The exact sequence is proprietary.
Epitope

ab32498 reacts with an epitope located in the N terminal region of Cyclin A2
Positive control
- WB: HeLa, K562 and Jurkat cell lysate. ICC/IF: HeLa cells. IP: K-562 cell lysate
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

E399
Isotype

IgG
Research areas

Western blot - Anti-Cyclin A2 antibody [E399] (ab32498)

All lanes : Anti-Cyclin A2 antibody [E399] (ab32498) at 1/1000 dilution

Lane 1 : HeLa whole cell lysate
Lane 2 : K562 whole cell lysate
Lane 3 : Jurkat whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 48 kDa
Observed band size: 50 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.

The lower band is unidentified.
Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [E399] (ab32498)

Immunofluorescent analysis of cyclin A expression in HeLa cells using 1/250 ab32498.
Immunoprecipitation - Anti-Cyclin A2 antibody [E399] (ab32498)

Purified ab32498 at 1/20 dilution (1µg) immunoprecipitating Cyclin A2 in K-562 whole cell lysate.
Lane 1 (input): K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10µg
Lane 2 (+): ab32498 + K-562 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32498 in K-562 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 50 kDa
Anti-Cyclin A2 antibody [E399] (ab32498)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com