Anti-Nanog antibody (ab21624)
Key features and details
- Rabbit polyclonal to Nanog
- Suitable for: ICC/IF, WB, Sandwich ELISA
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Nanog antibody
See all Nanog primary antibodies -
Description
Rabbit polyclonal to Nanog -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB MouseHuman
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Immunogen
Synthetic peptide corresponding to Human Nanog aa 29-49.
Sequence:CGPEENYPSLQMSSAEMPHTE
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General notes
Concentration of Anti-Nanog antibody: 0.2 mg/mL
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody (ab21624)ab21624 at a 1/1000 dilution staining Nanog in human embryonic stem cells by ICC. An antibody towards Oct4, which has an identical staining pattern to ab21624, was used as a positive control.
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Western blot - Anti-Nanog antibody (ab21624)All lanes : Anti-Nanog antibody (ab21624) at 1/200 dilution
Lane 1 : Human fetal fibroblast lysate
Lane 2 : Human iPS cell lysate
Predicted band size: 34 kDa
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Western blot - Anti-Nanog antibody (ab21624)All lanes : Anti-Nanog antibody (ab21624) at 1/1000 dilution
Lane 1 : whole cell extract of mouse 3T3 cells expressing mouse Nanog.
Lane 2 : whole cell extract of mouse 3T3 cells expressing human Nanog.
Predicted band size: 34 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
ab21624 recognizes human Nanog but does not recognize mouse Nanog in Western Blot. -
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody (ab21624) Image courtesy of Mick WauchopeImmunocytochemical analysis of Human iPSCs labeling Nanog with ab21624 at 1/75 dilution.
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Sandwich ELISA - Anti-Nanog antibody (ab21624)Standard Curve for Nanog (Analyte: Nanog protein (ab50053)); dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [1E6C4] to Nanog (ab76586) at 0.2 ug/ml and Detector Antibody Rabbit polyclonal to Nanog - ChIP Grade (ab21624) at 0.5 ug/ml.
