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Developmental Biology Embryogenesis Embryonic stem cells Intracellular

Anti-Dppa4 antibody (ab31648)

Price and availability

244 579 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-Dppa4 antibody (ab31648)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Dppa4
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Dppa4 antibody
    See all Dppa4 primary antibodies
  • Description

    Rabbit polyclonal to Dppa4
  • Host species

    Rabbit
  • Specificity

    The immunogen was designed to raise an antibody against mouse Dppa4. However, the antibody does not recognise a band of the expected size of Dppa4 in the mouse cell lysate tested. This may be because Dppa4 is not expressed in sufficient amounts for detection by Western Blotting in those cells. The immunogen also contained a sequence of 12 amino acids found in human Dppa4. The antibody recognises a band of approximately the expected size of Dppa4 in human embryonic stem cell lysate. Additionally, the immuogen contains a sequence of 12 consecutive amino acids which are found in human Dppa2 and a sequence of 13 amino acids of which 11 are found in mouse Dppa2. Therefore, it cannot be ruled out that the antibody recognizes Dppa2.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Mouse Dppa4 aa 200 to the C-terminus.
    (Peptide available as ab31647)

  • Positive control

    • Human Embryonic Stem Cell Lysate; Mouse Embryonic Stem Cell Lysate;

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Stem Cells
    • Embryonic Stem Cells
    • Intracellular
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Surface molecules

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Dppa4 protein (ab167860)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab31648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).

Target

  • Function

    May be involved in the maintenance of active epigenetic status of target genes (By similarity). May inhibit differentiation of embryonic cells into a primitive ectoderm lineage.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q7L190 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 55211 Human
    • Entrez Gene: 73693 Mouse
    • Omim: 614125 Human
    • SwissProt: Q7L190 Human
    • SwissProt: Q8CCG4 Mouse
    • Unigene: 317659 Human
    • Unigene: 35597 Mouse
    • Alternative names

      • 2410091M23Rik antibody
      • Developmental pluripotency associated 4 antibody
      • Developmental pluripotency associated protein 4 antibody
      • Developmental pluripotency-associated protein 4 antibody
      • Dppa 4 antibody
      • DPPA4 antibody
      • DPPA4_HUMAN antibody
      • FLJ10713 antibody
      see all

    Images

    • Western blot - Anti-Dppa4 antibody (ab31648)
      Western blot - Anti-Dppa4 antibody (ab31648)
      All lanes : Anti-Dppa4 antibody (ab31648) at 1 µg/ml

      Lane 1 : Human Embryonic Stem Cell Lysate
      Lane 2 : Mouse Embryonic Stem Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

      Predicted band size: 33 kDa
      Observed band size: 36 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 48 kDa (possible IgG), 48 kDa (possible glycosylated form), 48 kDa (possible non-specific binding)



      ab31648 recognized a band of approximately the predicted molecular weight (34 kDa) of Dppa4 in human, but not in mouse, embryonic stem cell lysates. The identity of the ~ 50 kDa band is not known.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab31648? Please let us know so that we can cite the reference in this datasheet.

    ab31648 has been referenced in 1 publication.

    • Takahashi K  et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861-72 (2007). WB ; Human . PubMed: 18035408

    Images

    • Western blot - Anti-Dppa4 antibody (ab31648)
      Western blot - Anti-Dppa4 antibody (ab31648)
      All lanes : Anti-Dppa4 antibody (ab31648) at 1 µg/ml

      Lane 1 : Human Embryonic Stem Cell Lysate
      Lane 2 : Mouse Embryonic Stem Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

      Predicted band size: 33 kDa
      Observed band size: 36 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 48 kDa (possible IgG), 48 kDa (possible glycosylated form), 48 kDa (possible non-specific binding)



      ab31648 recognized a band of approximately the predicted molecular weight (34 kDa) of Dppa4 in human, but not in mouse, embryonic stem cell lysates. The identity of the ~ 50 kDa band is not known.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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