Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Thursday March 04, 2021

Anti-Nanog antibody [EPR19646] (ab203919)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19646] to Nanog
Suitable for: IP, WB
Reacts with: Mouse, Human

Product name

Anti-Nanog antibody [EPR19646]
See all Nanog primary antibodies
Description

Rabbit monoclonal [EPR19646] to Nanog
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Mouse

WB
Mouse

Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: F9, NT2/D1 and NCCIT whole cell lysates. IP: F9 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Mouse
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19646
Isotype

IgG
Research areas

Western blot - Anti-Nanog antibody [EPR19646] (ab203919)

All lanes : Anti-Nanog antibody [EPR19646] (ab203919) at 1/1000 dilution

Lane 1 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 34 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

Nanog is expressed in embryonic stem cells, but not in in many somatic organs, fibroblast, lymphoid cells, and hematopoietic cell lines.

Negative control: NIH/3T3 (PMID:12787505).
Western blot - Anti-Nanog antibody [EPR19646] (ab203919)

All lanes : Anti-Nanog antibody [EPR19646] (ab203919) at 1/1000 dilution

Lane 1 : NT2/D1 (Human malignant pluripotent embryonic carcinoma cell line) whole cell lysate
Lane 2 : NCCIT (Human pluripotent embryonic carcinoma cell line) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 34 kDa
Observed band size: 42 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

Nanog is expressed in embryonic stem cells, but not in in many somatic organs, fibroblast, lymphoid cells, and hematopoietic cell lines.

Negative control: Jurkat (PMID:12787505).
Immunoprecipitation - Anti-Nanog antibody [EPR19646] (ab203919)

Nanog was immunoprecipitated from 0.35 mg of F9 (Mouse embryonic testicular cancer cell line) whole cell lysate with ab203919 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab203919 at 1/500 dilution.

VeriBlot for IP Detection Reaction (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: F9 whole cell lysate 10µg (Input).

Lane 2: ab203919 IP in F9 whole cell lysate.

Lane 3: Rabbit IgG,monoclonal [EPR25A]- Isotype Control (ab172730) instead of ab203919 in F9 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Anti-Nanog antibody [EPR19646] (ab203919)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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