Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Nanog antibody [EPR19646] (ab203919)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19646] to Nanog
  • Suitable for: IP, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Nanog antibody [EPR19646]
    See all Nanog primary antibodies

  • Description

    Rabbit monoclonal [EPR19646] to Nanog

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: F9, NT2/D1 and NCCIT whole cell lysates. IP: F9 whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Nanog antibody [EPR19646] (ab203919)
    Western blot - Anti-Nanog antibody [EPR19646] (ab203919)
    All lanes : Anti-Nanog antibody [EPR19646] (ab203919) at 1/1000 dilution

    Lane 1 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 34 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Nanog is expressed in embryonic stem cells, but not in in many somatic organs, fibroblast, lymphoid cells, and hematopoietic cell lines.

    Negative control: NIH/3T3 (PMID:12787505).

  • Western blot - Anti-Nanog antibody [EPR19646] (ab203919)
    Western blot - Anti-Nanog antibody [EPR19646] (ab203919)
    All lanes : Anti-Nanog antibody [EPR19646] (ab203919) at 1/1000 dilution

    Lane 1 : NT2/D1 (Human malignant pluripotent embryonic carcinoma cell line) whole cell lysate
    Lane 2 : NCCIT (Human pluripotent embryonic carcinoma cell line) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 34 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Nanog is expressed in embryonic stem cells, but not in in many somatic organs, fibroblast, lymphoid cells, and hematopoietic cell lines.

    Negative control: Jurkat (PMID:12787505).

  • Immunoprecipitation - Anti-Nanog antibody [EPR19646] (ab203919)
    Immunoprecipitation - Anti-Nanog antibody [EPR19646] (ab203919)

    Nanog was immunoprecipitated from 0.35 mg of F9 (Mouse embryonic testicular cancer cell line) whole cell lysate with ab203919 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab203919 at 1/500 dilution.

    VeriBlot for IP Detection Reaction (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: F9 whole cell lysate 10µg (Input).

    Lane 2: ab203919 IP in F9 whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A]- Isotype Control (ab172730) instead of ab203919 in F9 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Anti-Nanog antibody [EPR19646] (ab203919)
    Anti-Nanog antibody [EPR19646] (ab203919)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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