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Developmental Biology Embryogenesis Embryonic stem cells Intracellular

Anti-Nanog antibody (ab70482)

Price and availability

308 236 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-Nanog antibody (ab70482)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Nanog
  • Suitable for: WB, IP
  • Reacts with: Mouse
  • Isotype: IgG

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Overview

  • Product name

    Anti-Nanog antibody
    See all Nanog primary antibodies
  • Description

    Rabbit polyclonal to Nanog
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    See all applications and species data
  • Immunogen

    A region between residue 250 and the C-terminus (residue 305) of Mouse Nanog using the numbering given in entry NP_079141.2

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Stem Cells
    • Embryonic Stem Cells
    • Intracellular
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • SMADs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Cell cycle
    • Cell differentiation
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Surface molecules

Images

  • Western blot - Anti-Nanog antibody (ab70482)
    Western blot - Anti-Nanog antibody (ab70482)
    All lanes : Anti-Nanog antibody (ab70482) at 0.1 µg/ml

    Lane 1 : Whole cell lysate
    from mouse embryonic stem cells at 15 µg
    Lane 2 : Whole cell lysate
    from mouse embryonic stem cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 35 kDa
    Observed band size: 39 kDa
    why is the actual band size different from the predicted?


    Exposure time: 12 seconds
  • Immunoprecipitation - Anti-Nanog antibody (ab70482)
    Immunoprecipitation - Anti-Nanog antibody (ab70482)
    Detection of Mouse Nanog by Western Blot of Immunoprecipitation with whole cell lysate (1mg) from mouse embryonic stem cells and ab 70482 at 2&microg/mg lysate. Lane 1: ab70482, Lane 2: rabbit IgG control Immunoprecipitated Nanog was blotted using an anti-Nanog antibody at 0.1&microg/ml.
  • Western blot - Anti-Nanog antibody (ab70482)
    Western blot - Anti-Nanog antibody (ab70482) This image is courtesy of an anonymous Abreview
    All lanes : Anti-Nanog antibody (ab70482) at 1/1000 dilution (for 16 hours at 4°C)

    Lane 1 : Murine Embryonic Fibroblasts (MEFs)
    Lane 2 : Murine Embryonic Stem (ES) Cells

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : An HRP-conjugated Donkey anti-rabbit IgG monoclonal at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 35 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking Step: 3% BSA for 1 hour at 20°C

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Anti-Nanog antibody [EPR20694] - ChIP Grade (ab214549)

    Applications: ChIP, Flow Cyt, ICC/IF, IHC-P, IP, WB

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    Anti-Nanog antibody [EPR19646] (ab203919)

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