Anti-Nanog antibody (ab70482)
Key features and details
- Rabbit polyclonal to Nanog
- Suitable for: WB, IP
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Nanog antibody
See all Nanog primary antibodies -
Description
Rabbit polyclonal to Nanog -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseWB Mouse -
Immunogen
A region between residue 250 and the C-terminus (residue 305) of Mouse Nanog using the numbering given in entry NP_079141.2
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Nanog antibody (ab70482) at 0.1 µg/ml
Lane 1 : Whole cell lysate
from mouse embryonic stem cells at 15 µg
Lane 2 : Whole cell lysate
from mouse embryonic stem cells at 50 µg
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Exposure time: 12 seconds
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Detection of Mouse Nanog by Western Blot of Immunoprecipitation with whole cell lysate (1mg) from mouse embryonic stem cells and ab 70482 at 2µg/mg lysate. Lane 1: ab70482, Lane 2: rabbit IgG control Immunoprecipitated Nanog was blotted using an anti-Nanog antibody at 0.1µg/ml.
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All lanes : Anti-Nanog antibody (ab70482) at 1/1000 dilution (for 16 hours at 4°C)
Lane 1 : Murine Embryonic Fibroblasts (MEFs)
Lane 2 : Murine Embryonic Stem (ES) Cells
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : An HRP-conjugated Donkey anti-rabbit IgG monoclonal at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Blocking Step: 3% BSA for 1 hour at 20°C