Anti-IGF1 antibody (ab106836)
Key features and details
- Goat polyclonal to IGF1
- Suitable for: WB, ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IGF1 antibody
See all IGF1 primary antibodies -
Description
Goat polyclonal to IGF1 -
Host species
Goat -
Tested applications
Suitable for: WB, ICC, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig
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Immunogen
Peptide with sequence
C-RSVRAQRHTD
, from the internal region of human IGF1 sequence according to NP_001104753.1; NP_001104754.1; NP_001104755.1; NP_000609.1. -
Positive control
- ICC: Human HEK-293 cells; WB: Human uterus tissue lysate; IHC-P: Human spleen tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IGF1 antibody (ab106836)All lanes : Anti-IGF1 antibody (ab106836) at 0.5 µg/ml
Lane 1 : Human uterus tissue lysate in RIPA buffer
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 22 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour at room temperature. Detected by chemiluminescence.
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Immunocytochemistry - Anti-IGF1 antibody (ab106836)Immunocytochemistry analysis of paraformaldehyde fixed HEK-293 (human epithelial cell line from embryonic kidney) cells, permeabilized with 0.15% Triton. Primary incubation for 1 hour at 10 µg/mL followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
