Anti-IGF1 antibody (ab223567)
Key features and details
- Rabbit polyclonal to IGF1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-IGF1 antibody
See all IGF1 primary antibodies -
Description
Rabbit polyclonal to IGF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human IGF1. From mid-protein of human IGF1.
Database link: P05019 -
Positive control
- WB: Mouse liver lysate. ICC/IF: RKO (human colon carcinoma cell line) cells. IHC-P: Human liver.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IGF1 antibody (ab223567)Anti-IGF1 antibody (ab223567) at 1/1000 dilution + mouse liver lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG HRP at 1/2000 dilution
Developed using the ECL technique.
Observed band size: 22 kDa why is the actual band size different from the predicted?
Exposure time: 6 minutesBlocking: 5% skimmed milk in 1X TBST.
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Immunocytochemistry/ Immunofluorescence - Anti-IGF1 antibody (ab223567)4% formaldehyde fixed RKO (human colon carcinoma cell line) cells stained for IGF1 (green) using ab223567 at a 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit 488 at a 1/100 dilution. Phalloidin Texas Red F-Actin 1/1000 (red) and DAPI 1/5000 (blue) were used as counterstains.
