Anti-IGF1 antibody (ab9572)
Key features and details
- Rabbit polyclonal to IGF1
- Suitable for: ICC/IF, IHC-FoFr, WB, Neutralising, IHC-P, Sandwich ELISA
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-IGF1 antibody
See all IGF1 primary antibodies -
Description
Rabbit polyclonal to IGF1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-FoFr, WB, Neutralising, IHC-P, Sandwich ELISAmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment -
Immunogen
Recombinant full length protein corresponding to Human IGF1. Highly pure (>98%) recombinant hIGF-1 (human Insulin Like Growth Factor-1).
Database link: P05019 -
Positive control
- IHC-P: Human pancreas and liver tissues.
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General notes
Although some customers have been successful in IHC we no longer batch test in this application. For an IHC validated antibody please see ab263903
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 antibody (ab9572)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling IGF1 with ab9572 at 1 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
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Sandwich ELISA - Anti-IGF1 antibody (ab9572)To detect hIGF-I by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-I as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-I.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 antibody (ab9572)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling IGF1 with ab9572 at 1 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
