All lanes : Anti-IGF1 antibody (ab36532) at 1/2500 dilution

Lane 1 : Rat IGF1 at 1.5 µg
Lane 2 : Rat IGF1 at 0.75 µg
Lane 3 : Rat IGF1 at 0.375 µg
Lane 4 : Rat IGF1 at 0.1875 µg
Lane 5 : Rat IGF1 at 0.09375 µg

Secondary
All lanes : HRP-conjugated anti-mouse IgG at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 7.5 kDa