Anti-PEDF antibody (ab74439)
Key features and details
- Rabbit polyclonal to PEDF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PEDF antibody
See all PEDF primary antibodies -
Description
Rabbit polyclonal to PEDF -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human PEDF aa 1-100 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab74438) -
Positive control
- WB: Plasma total protein lysate. Human kidney tissue lysate - total protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications ChimpanzeeApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 46, 48 kDa (predicted molecular weight: 46 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 46, 48 kDa (predicted molecular weight: 46 kDa).Target
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Function
Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. -
Tissue specificity
Retinal pigment epithelial cells and blood plasma. -
Sequence similarities
Belongs to the serpin family. -
Developmental stage
Expressed in quiescent cells. -
Domain
The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. -
Post-translational
modificationsThe N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
Cellular localization
Secreted. Melanosome. Enriched in stage I melanosomes. - Information by UniProt
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Database links
- Entrez Gene: 5176 Human
- Omim: 172860 Human
- SwissProt: P36955 Human
- Unigene: 532768 Human
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Alternative names
- Cell proliferation-inducing gene 35 protein antibody
- EPC 1 antibody
- EPC-1 antibody
see all
Images
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Western blot - Anti-PEDF antibody (ab74439)All lanes : Anti-PEDF antibody (ab74439) at 1 µg/ml
Lane 1 : Human plasma total protein lysate
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 10 µg per lane.
Secondary
Lane 1 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Lane 2 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Additional bands at: 150 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
PEDF is known to be phosphorylated and glycosylated. This could explain the bands appearing higher than the expected molecular weight.
Datasheets and documents
References (0)
ab74439 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-PEDF antibody (ab74439)All lanes : Anti-PEDF antibody (ab74439) at 1 µg/ml
Lane 1 : Human plasma total protein lysate
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 10 µg per lane.
Secondary
Lane 1 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Lane 2 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Additional bands at: 150 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
PEDF is known to be phosphorylated and glycosylated. This could explain the bands appearing higher than the expected molecular weight.
