Anti-PEDF antibody (ab14993)
Key features and details
- Rabbit polyclonal to PEDF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PEDF antibody
See all PEDF primary antibodies -
Description
Rabbit polyclonal to PEDF -
Host species
Rabbit -
Specificity
Recognizes PEDF at 50kDa. Additional bands were detected at 66 and 76kDa, which were competed away by immunogen. These bands may be post translationally modified forms. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Fusion protein (His-tag) corresponding to Human PEDF. Corresponding to full length human PEDF expressed in baby hamster kidney cells.
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Positive control
- WB: RIPA lysates from Y79 cells.
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General notes
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.184% Tris glycine, 30% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PEDF antibody (ab14993) Kindly submitted by Dr John Wardale.
Lanes 1-4: Cartilage lysates from 4 osteoarthritic knee replacement donors (100 mg (wet weight) powdered cartilage/ 1 ml RIPA buffer, extracted for 24 hrs at 4°C)
Lane C: Positive control (overexpressing cell line)
Anticipated band at approx 50kDa detected. Specificity of antibody allowed relatively long exposure and quite high concentrations of primary and secondary antibodies to be used with negligible background. Good results were also obtained with unreduced samples.
The additional approx 250kDa band is likely to be due to PEDF crosslinked or bound to high molecular weight matrix proteins (collagen, aggrecan) which are present in cartilage lysates. Reduction using beta mercaptoethanol reduced some, but not all high MW staining and increased the intensity of the 50kDa band. -
Western blot - Anti-PEDF antibody (ab14993)Anti-PEDF antibody (ab14993) at 1 µg/ml + Y79 (Human retinoblastoma cell line) cell lysate in RIPA buffer at 40 µg
Secondary
IgG (HRP) goat anti-rabbit
Predicted band size: 50 kDaChemiluminescence detection system.
