Anti-MDM2 antibody [SMP 14] (ab3110)
![Anti-MDM2 antibody [SMP 14] (ab3110)](https://www.abcam.com/ps/products/3/ab3110/Images/ab3110-383450-anti-mdm2-antibody-smp-14-western-blot-hepg2-nutlin3a10um-24hr-hepg2-nutlin3a10um-24hr.jpg "Anti-MDM2 antibody [SMP 14] (ab3110)")
Key features and details
- Mouse monoclonal [SMP 14] to MDM2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-MDM2 antibody [SMP 14]
See all MDM2 primary antibodies -
Description
Mouse monoclonal [SMP 14] to MDM2 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human MDM2 aa 150-250. Cross-linked to KLH using glutaldehyde.
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Epitope
Amino acids 154 - 167. -
Positive control
- HepG2 cell lysate (treated with 10uM Nutlin-3a for 24hrs)
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
SMP 14 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-MDM2 antibody [SMP 14] (ab3110)All lanes : Anti-MDM2 antibody [SMP 14] (ab3110) at 2 µg/ml
Lane 1 : HepG2 cell lysate
Lane 2 : HepG2 treated with 10uM Nutlin-3a for 24hrs cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab3110 observed at 75 kDa. Red - loading control, ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.
ab3110 was shown to react with MDM2 in cell lysates subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab3110 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at 2 µg/ml and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
