Anti-MDM2 (phospho S166) antibody (ab131355)
Key features and details
- Rabbit polyclonal to MDM2 (phospho S166)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-MDM2 (phospho S166) antibody
See all MDM2 primary antibodies -
Description
Rabbit polyclonal to MDM2 (phospho S166) -
Host species
Rabbit -
Specificity
ab131355 detects endogenous level of MDM2 only when phosphorylated at Serine 166. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic phosphopeptide conjugated to KLH, surrounding phosphorylation site of Serine 166 (
A-I-S(p)-E-T
) derived from Human MDM2 (NP_002383.2). -
Positive control
- 293 cell extract treated with Hydroxyurea, HeLa cells, Human breast carcinoma tissue
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab131355 is purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-MDM2 (phospho S166) antibody (ab131355) at 1/500 dilution
Lane 1 : 293 cell extract untreated
Lane 2 : 293 cell extract treated with hydroxyurea
Predicted band size: 55 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
-
Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling MDM2 (phospho S166) with ab131355 at a dilution of 1/50. Picture on the right was preincubated with blocking peptide.
-
Immunofluorescence of methanol-fixed HeLa cells staining MDM2 (phospho S166) with ab131355 at a dilution of 1/100.