Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Survivin antibody [EPR20448] - BSA and Azide free (ab231076)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20448] to Survivin - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Survivin antibody [EPR20448] - BSA and Azide free
    See all Survivin primary antibodies

  • Description

    Rabbit monoclonal [EPR20448] to Survivin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: A549 cells.

  • General notes

    Ab231076 is the carrier-free version of ab208938. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab231076 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-Survivin antibody [EPR20448] - BSA and Azide free (ab231076)
    Immunoprecipitation - Anti-Survivin antibody [EPR20448] - BSA and Azide free (ab231076)

    Survivin was immunoprecipitated from 0.35mg of A549 (human lung carcinoma cell line) whole cell lysate with ab208938 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab208938 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: A549 whole cell lysate 10 µg (Input). 

    Lane 2: ab208938 IP in A549 whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208938 in A549 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208938).

  • Flow Cytometry - Anti-Survivin antibody [EPR20448] - BSA and Azide free (ab231076)
    Flow Cytometry - Anti-Survivin antibody [EPR20448] - BSA and Azide free (ab231076)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized A549 (human lung carcinoma cell line) cell line labeling Survivin with ab208938 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208938).

  • Flow Cytometry - Anti-Survivin antibody [EPR20448] - BSA and Azide free (ab231076)
    Flow Cytometry - Anti-Survivin antibody [EPR20448] - BSA and Azide free (ab231076)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Survivin with ab208938 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208938).

  • Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody [EPR20448] - BSA and Azide free (ab231076)
    Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody [EPR20448] - BSA and Azide free (ab231076)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Survivin with ab208938 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing kinetochores and midbody (arrow) staining in the HeLa cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)  (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208938).

  • Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody [EPR20448] - BSA and Azide free (ab231076)
    Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody [EPR20448] - BSA and Azide free (ab231076)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells labeling Survivin with ab208938 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing midbody (arrow) staining in the A549 cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)  (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208938).

  • Anti-Survivin antibody [EPR20448] - BSA and Azide free (ab231076)
    Anti-Survivin antibody [EPR20448] - BSA and Azide free (ab231076)

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