Anti-Survivin antibody [EPR2675] (ab134170)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2675] to Survivin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Survivin antibody [EPR2675]
See all Survivin primary antibodies -
Description
Rabbit monoclonal [EPR2675] to Survivin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatHumanIHC-P MouseRatHumanWB MouseRatHuman -
Immunogen
Synthetic peptide within Human Survivin aa 50-150. The exact sequence is proprietary.
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Positive control
- Human cervical carcinoma tissue; HeLa and MOLT4 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2675 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Survivin antibody [EPR2675] (ab134170) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MOLT4 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 16 kDaSecondary antibody - anti-rabbit HRP (ab6721)
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All lanes : Anti-Survivin antibody [EPR2675] (ab134170) at 1/300 dilution
Lane 1 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 16 kDa
Exposure time: 1 minuteBlocking and diluting buffer: 5% NFDM/TBST.
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Ab134170 staining Survivin in paraffin-embedded rat spleen tissue sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin embedded sections). Samples are incubated in primary antibody at 1:300 dilution (3µg/ml). A Goat Anti-rabbit IgG H&L (HRP) (ab97051) was used as the secondary antibody at 1:500 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Hematoxylin was used as a counterstain. Sporadically nuclear staining on rat spleen.
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Ab134170 staining Survivin in C6 (rat glial tumor glial cell) cells by Immunocytochemistry. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1:500 dilution (2.5µg/ml). An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1:1000 dilution (2µg/ml). Cells were counterstained with an Alexa Fluor® 594 Anti- Alpha Tubulin [DM1A]- Microtubule Marker at 1:200 dilution (2.5µg/ml). DAPI was used as a nuclear counterstain. Confocal image showing strong midbody (arrow) staining in C6 cell line.
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Ab134170 staining Survivin in paraffin-embedded mouse stomach tissue sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin embedded sections). Samples are incubated in primary antibody at 1:300 dilution (3µg/ml). A Goat Anti-rabbit IgG H&L (HRP) (ab97051) was used as the secondary antibody at 1:500 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Hematoxylin was used as a counterstain. Sporadically nuclear staining on mouse stomach.
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Ab134170 staining Survivin in MCF7 (human breast adenocarcinoma epithelial cell line) cells by Immunocytochemistry. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1:500 dilution (2.5µg/ml). An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1:1000 dilution (2µg/ml). Cells were counterstained with an Alexa Fluor® 594 Anti- Alpha Tubulin [DM1A]- Microtubule Marker at 1:200 dilution (2.5µg/ml). DAPI was used as a nuclear counterstain. Confocal image showing strong midbody (arrow) staining in MCF7 cell line.
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Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue, staining Survivin using ab134170 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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