Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2675] to Survivin - BSA and Azide free
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Survivin antibody [EPR2675] - BSA and Azide free
    See all Survivin primary antibodies

  • Description

    Rabbit monoclonal [EPR2675] to Survivin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab167450 is the carrier-free version of ab134170. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab167450 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
    Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)

    Ab134170 staining Survivin in C6 (rat glial tumor glial cell) cells by Immunocytochemistry. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1:500 dilution (2.5µg/ml). An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1:1000 dilution (2µg/ml). Cells were counterstained with an Alexa Fluor® 594 Anti- Alpha Tubulin [DM1A]- Microtubule Marker at 1:200 dilution (2.5µg/ml). DAPI was used as a nuclear counterstain. Confocal image showing strong midbody (arrow) staining in C6 cell line.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134170).

  • Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
    Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)

    Ab134170 staining Survivin in MCF7 (human breast adenocarcinoma epithelial cell line) cells by Immunocytochemistry. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1:500 dilution (2.5µg/ml). An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1:1000 dilution (2µg/ml). Cells were counterstained with an Alexa Fluor® 594 Anti- Alpha Tubulin [DM1A]- Microtubule Marker at 1:200 dilution (2.5µg/ml). DAPI was used as a nuclear counterstain. Confocal image showing strong midbody (arrow) staining in MCF7 cell line. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134170).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)

    Ab134170 staining Survivin in paraffin-embedded rat spleen tissue sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin embedded sections). Samples are incubated in primary antibody at 1:300 dilution (3µg/ml). A Goat Anti-rabbit IgG H&L (HRP) (ab97051) was used as the secondary antibody at 1:500 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Hematoxylin was used as a counterstain. Sporadically nuclear staining on rat spleen. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134170).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)

    Ab134170 staining Survivin in paraffin-embedded mouse stomach tissue sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin embedded sections). Samples are incubated in primary antibody at 1:300 dilution (3µg/ml). A Goat Anti-rabbit IgG H&L (HRP) (ab97051) was used as the secondary antibody at 1:500 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Hematoxylin was used as a counterstain. Sporadically nuclear staining on mouse stomach.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134170).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)

    Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue, staining Survivin using ab134170 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134170).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
    Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)

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