Anti-S100A4 antibody (ab58597)
Key features and details
- Goat polyclonal to S100A4
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-S100A4 antibody
See all S100A4 primary antibodies -
Description
Goat polyclonal to S100A4 -
Host species
Goat -
Specificity
Both variants (NP_002952.1; NP_062427.1) represent identical protein. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide:
CNEFFEGFPDKQP
, corresponding to C terminal amino acids 86-98 of Human S100A4 -
Positive control
- Human Placenta lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-S100A4 antibody (ab58597)Anti-S100A4 antibody (ab58597) at 0.03 µg/ml + Peripheral blood mononuclear cell lysate (in RIPA buffer) at 35 µg
Predicted band size: 12 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody (ab58597)ab58597 at a concentration of 2µg/ml staining S100A4 in lymphatic cells in the lamina propria of human small intestine tissue sections by immunohistochemistry (paraffin-embedded). Antigen retrieval was with a citrate buffer.
