Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-S100A4 antibody [EPR14639(2)] - BSA and Azide free (ab220213)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14639(2)] to S100A4 - BSA and Azide free
  • Suitable for: IP, Flow Cyt, ICC/IF, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-S100A4 antibody [EPR14639(2)] - BSA and Azide free
    See all S100A4 primary antibodies

  • Description

    Rabbit monoclonal [EPR14639(2)] to S100A4 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Based on sequence homologies, the antibody may cross-react with other proteins of the same family (S100A1-12). We did not perform any experiments to confirm this.

    We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Rat
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, A549, and A375 cell lysates and human fetal spleen tissue lysates. IHC-P: Human cervix carcinoma, lung carcinoma and gastric carcinoma tissues. ICC/IF: Jurkat cells. Flow Cyt: Jurkat cells.

  • General notes

    ab220213 is the carrier-free version of ab197896. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab220213 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-S100A4 antibody [EPR14639(2)] - BSA and Azide free (ab220213)
    Western blot - Anti-S100A4 antibody [EPR14639(2)] - BSA and Azide free (ab220213)
    All lanes : Anti-S100A4 antibody [EPR14639(2)] (ab197896) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : S100A4 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 12 kDa
    Observed band size: 12 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab197896).

    Lanes 1 - 2: Merged signal (red and green). Green - ab197896 observed at 12 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab197896 Recombinant Anti-S100A4 antibody [EPR14639(2)] was shown to specifically react with S100A4 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261758 (knockout cell lysate ab257045) was used. Wild-type and S100A4 knockout samples were subjected to SDS-PAGE. ab197896 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR14639(2)] - BSA and Azide free (ab220213)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR14639(2)] - BSA and Azide free (ab220213)

    Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling S100A4 using ab197896 at 1/2000 dilution. Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used ay 1/500 dilution as a secondary antibody and cells were counterstained with Hematoxylin.

    Inset image: negative control obtained using PBS instead of ab197896 and secondary antibody only.
    Note: Nuclear and cytoplasm staining on cervix carcinoma tissue was observed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197896).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-S100A4 antibody [EPR14639(2)] - BSA and Azide free (ab220213)
    Immunoprecipitation - Anti-S100A4 antibody [EPR14639(2)] - BSA and Azide free (ab220213)

    ab197896 at 1/40 immunoprecipitating S100A4 in A549 whole cell lysate observed at 12 KDa.

    Lane 1 (input): A549 whole cell lysate 10μg

    Lane 2 (+): ab197896 + A549 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab197896 in A549 whole cell lysate

    For western blotting, Panel A: ab197896, 1:1000; Panel B: ab124805, 1:1000 and anti-rabbit IgG (HRP), specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197896).

  • Immunoprecipitation - Anti-S100A4 antibody [EPR14639(2)] - BSA and Azide free (ab220213)
    Immunoprecipitation - Anti-S100A4 antibody [EPR14639(2)] - BSA and Azide free (ab220213)

    ab197896 at 1/40 immunoprecipitating S100A4 in A549 whole cell lysate observed at 12 KDa.

    Lane 1 (+): ab197896 + A549 whole cell lysate.

    Lane 2 (-): Rabbit monoclonal IgG (ab172730) instead of ab197896 in A549 whole cell lysate

    For western blotting, ab197896 at 1/1000 and anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG (1/1500).

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197896).

  • Flow Cytometry - Anti-S100A4 antibody [EPR14639(2)] - BSA and Azide free (ab220213)
    Flow Cytometry - Anti-S100A4 antibody [EPR14639(2)] - BSA and Azide free (ab220213)

    Flow Cytometry analysis of Jurkat cells labelling S100A4 with ab197896 at 1/250 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197896).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR14639(2)] - BSA and Azide free (ab220213)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR14639(2)] - BSA and Azide free (ab220213)

    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling S100A4 using ab197896 at 1/2000 dilution. Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as a secondary antibody at a dilution of 1/500 and cells were counterstained with Hematoxylin.

    Inset image: negative control obtained using PBS instead of ab197896 and secondary antibody only.
    Note: Nuclear and weakly staining on lung carcinoma tissue was observed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197896).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR14639(2)] - BSA and Azide free (ab220213)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR14639(2)] - BSA and Azide free (ab220213)

    Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling S100A4 using ab197896 at 1/2000 dilution. Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as a secondary antibody at 1/500 dilution. Cells were counterstained with Hematoxylin. 

    Inset image: negative control obtained using PBS instead of ab197896 and secondary antibody only.
    Note: Cytoplasm and nuclear staining on human gastric carcinoma tissue was observed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197896).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-S100A4 antibody [EPR14639(2)] - BSA and Azide free (ab220213)
    Anti-S100A4 antibody [EPR14639(2)] - BSA and Azide free (ab220213)

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