Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2761(2)] to S100A4 - BSA and Azide free
  • Suitable for: WB, IP, ICC/IF, Flow Cyt, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free
    See all S100A4 primary antibodies

  • Description

    Rabbit monoclonal [EPR2761(2)] to S100A4 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Some optimisation may be required for detection of the target protein due to low levels of endogenous expression in some samples.  Please see images below for suitable positive controls.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Positive control

    • Human tonsil, A549, A375, HeLa and Human small intestine lysates; Human tonsil tissue

  • General notes

    ab216003 is the carrier-free version of ab124805 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab216003 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)

    ab124805 staining S100A4 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124805).

  • Flow Cytometry - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
    Flow Cytometry - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)

    ab124805 staining S100A4 in HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 80% methanol and permeabilised with 0.1% Triton X-100 (in PBS). The sample was incubated with the primary antibody at a dilution of 1/800. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: ab172730 rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124805).

  • Flow Cytometry - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
    Flow Cytometry - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)

    ab124805 staining S100A4 in HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 80% methanol and permeabilised with 0.1% Triton X-100 (in PBS). The sample was incubated with the primary antibody at a dilution of 1/80. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: ab172730 rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124805).

  • Immunoprecipitation - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
    Immunoprecipitation - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)

    ab124805 immunoprecipitating S100A4. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/30 and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a dilution of 1/1500.

    Lane 1: Human tonsil whole cell lysate (10ug)
    Lane 2: Human tonsil whole cell lysate 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab124805 in human tonsil whole cell lysate

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124805).

  • Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
    Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)

    ab124805 staining S100A4 in Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1/500. ab7291 and ab150120 were used as counterstains for primary antibody ab124805 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124805).

  • Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
    Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003) This image is courtesy of an anonymous Abreview.

    Unpurified ab124805 staining S100A4 in the A549 cell line from Human lungs by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with Triton X-100 0.25% in PBS. Samples were incubated with primary antibody (1/100) for 45 minutes at 25°C. A TRITC-conjugated Goat anti-rabbit  polyclonal was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124805).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)

    Unpurified ab124805, at 1/250 dilution, staining S100A4 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124805).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003) This image is courtesy of an anonymous Abreview.

    Immunohistochemical analysis of Human lung tissue, staining S100A4 with unpurified ab124805.

    Tissue was fixed with HOPE and blocked with blocking solution for 5 minutes at 25°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 25°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124805).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
    Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)

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