Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20918-13] to Casein Kinase 1 gamma 2
  • Suitable for: IP, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13]
    See all Casein Kinase 1 gamma 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20918-13] to Casein Kinase 1 gamma 2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    Human
    IP
    Mouse
    WB
    Mouse
    Rat
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with human Casein Kinase 1 gamma 2; HEK-293T transfected with mouse Casein Kinase 1 gamma 2; Mouse and rat testis lysate. Flow Cyt: MEF and HEK-293T cells. IP: Mouse testis lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)
    Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)
    All lanes : Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121) at 1/1000 dilution

    Lane 1 : Mouse testis tissue lysate
    Lane 2 : Rat testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
    Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051))

    Predicted band size: 47 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Flow Cytometry - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)
    Flow Cytometry - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol-permeabilized MEF (Mouse embryonic fibroblast (immortalized) cells labelling Casein Kinase 1 gamma 2 with ab238121 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)
    Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)
    All lanes : Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121) at 1/1000 dilution

    Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc- tag®, whole cell lysate
    Lane 2 : HEK-293T transfected with human Casein Kinase 1 gamma 2 (WT) expression vector containing a myc-tag®, whole cell lysate
    Lane 3 : HEK-293T transfected with mouse Casein Kinase 1 gamma 2 (WT) expression vector containing a myc-tag®, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 47 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

  • Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)
    Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)

    Casein Kinase 1 gamma 2 was immunoprecipitated from 0.35 mg mouse testis tissue lysate with ab238121 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238121 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse testis tissue lysate 10 ug

    Lane 2: ab238121 IP in mouse testis tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238121 in mouse testis tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32 seconds.

     

  • Flow Cytometry - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)
    Flow Cytometry - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol-permeabilized HEK-293T (Human embryonic kidney epithelial cell) cells (transfected with myc-tagged Casein Kinase 1 gamma 2 construct ) labelling Casein Kinase 1 gamma 2 with ab238121 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)
    Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)

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