Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PKN3 antibody [EPR3511(2)] (ab155076)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3511(2)] to PKN3
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PKN3 antibody [EPR3511(2)]
    See all PKN3 primary antibodies

  • Description

    Rabbit monoclonal [EPR3511(2)] to PKN3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PKN3 aa 100-200. The exact sequence is proprietary.
    Database link: Q6P5Z2

  • Positive control

    • WB: PC-3, Mouse spleen, Rat spleen lysates ICC/IF: LNCaP cells. Flow Cyt: LNCaP cells.

  • General notes

     

     

     This product was previously labelled as PKN beta

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR3511(2)

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PKN3 antibody [EPR3511(2)] (ab155076)
    Immunocytochemistry/ Immunofluorescence - Anti-PKN3 antibody [EPR3511(2)] (ab155076)

    Immunocytochemistry/ Immunofluorescence analysis of LNCaP (Human prostate carcinoma epithelial cell) cells labeling PKN3 with Purified ab155076 at 1:300 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-PKN3 antibody [EPR3511(2)] (ab155076)
    Flow Cytometry - Anti-PKN3 antibody [EPR3511(2)] (ab155076)

    Flow Cytometry analysis of LNCaP (Human prostate carcinoma epithelial cell) cells labeling PKN3 with Purified ab155076 at 1:300 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-PKN3 antibody [EPR3511(2)] (ab155076)
    Western blot - Anti-PKN3 antibody [EPR3511(2)] (ab155076)
    All lanes : Anti-PKN3 antibody [EPR3511(2)] (ab155076) at 1/5000 dilution (Purified)

    Lane 1 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Mouse spleen lysates
    Lane 3 : Rat spleen lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 99 kDa
    Observed band size: 106 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-PKN3 antibody [EPR3511(2)] (ab155076)
    Western blot - Anti-PKN3 antibody [EPR3511(2)] (ab155076)
    All lanes : Anti-PKN3 antibody [EPR3511(2)] (ab155076) at 1/1000 dilution

    Lane 1 : LNCaP cell lysate
    Lane 2 : PC-3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 99 kDa



    This image was generated using the unpurified version of the product. 

  • Anti-PKN3 antibody [EPR3511(2)] (ab155076)
    Anti-PKN3 antibody [EPR3511(2)] (ab155076)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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