Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PKN3 antibody [EPR3511(2)] (ab155076)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3511(2)] to PKN3
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-PKN3 antibody [EPR3511(2)]
See all PKN3 primary antibodies
Description

Rabbit monoclonal [EPR3511(2)] to PKN3
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human PKN3 aa 100-200. The exact sequence is proprietary.
Database link: Q6P5Z2
Positive control
- WB: PC-3, Mouse spleen, Rat spleen lysates ICC/IF: LNCaP cells. Flow Cyt: LNCaP cells.
General notes
This product was previously labelled as PKN beta

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3511(2)
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-PKN3 antibody [EPR3511(2)] (ab155076)

Immunocytochemistry/ Immunofluorescence analysis of LNCaP (Human prostate carcinoma epithelial cell) cells labeling PKN3 with Purified ab155076 at 1:300 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry - Anti-PKN3 antibody [EPR3511(2)] (ab155076)

Flow Cytometry analysis of LNCaP (Human prostate carcinoma epithelial cell) cells labeling PKN3 with Purified ab155076 at 1:300 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-PKN3 antibody [EPR3511(2)] (ab155076)

All lanes : Anti-PKN3 antibody [EPR3511(2)] (ab155076) at 1/5000 dilution (Purified)

Lane 1 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse spleen lysates
Lane 3 : Rat spleen lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 99 kDa
Observed band size: 106 kDa
why is the actual band size different from the predicted?
Western blot - Anti-PKN3 antibody [EPR3511(2)] (ab155076)

All lanes : Anti-PKN3 antibody [EPR3511(2)] (ab155076) at 1/1000 dilution

Lane 1 : LNCaP cell lysate
Lane 2 : PC-3 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 99 kDa

This image was generated using the unpurified version of the product.
Anti-PKN3 antibody [EPR3511(2)] (ab155076)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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