Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

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Anti-TFPI antibody [EPR22977-133] (ab260042)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22977-133] to TFPI
Suitable for: WB, ICC/IF, IP
Reacts with: Human

Product name

Anti-TFPI antibody [EPR22977-133]
See all TFPI primary antibodies
Description

Rabbit monoclonal [EPR22977-133] to TFPI
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human placenta lysate. HepG2, Caco-2, U-87 MG, HUVEC and HT-29 lysates. ICC/IF: HepG2 and HUVEC cells. IP: HepG2 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22977-133
Isotype

IgG
Research areas

Western blot - Anti-TFPI antibody [EPR22977-133] (ab260042)

All lanes : Anti-TFPI antibody [EPR22977-133] (ab260042) at 1/1000 dilution

Lane 1 : Human placenta lysate
Lane 2 : Human colon lysate
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 4 : Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 5 : U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate
Lane 6 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 7 : HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 8 : LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 3-8 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 35 kDa
Observed band size: 45,47 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22144186).

Negative control: Human colon and LoVo (PMID: 20062932, 9311608).

Exposure times: Lanes 1-2: 59 seconds; Lane 3: 26 seconds; Lanes 4-8: 59 seconds.
Immunoprecipitation - Anti-TFPI antibody [EPR22977-133] (ab260042)

TFPI was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg with ab260042 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260042 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

Lane 1: HepG2 whole cell lysate 10µg

Lane 2: ab260042 IP in HepG2 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab260042 in HepG2 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.
Immunocytochemistry/ Immunofluorescence - Anti-TFPI antibody [EPR22977-133] (ab260042)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC (human umbilical vein endothelial cell) cells labeling TFPI with ab260042 at 1/100 (6.8 µg/ml) dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing cytoplasmic staining in HUVEC cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^®594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-TFPI antibody [EPR22977-133] (ab260042)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling TFPI with ab260042 at 1/100 (6.8 µg/ml) dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing cytoplasmic and membranous staining in HepG2 cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^®594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
Anti-TFPI antibody [EPR22977-133] (ab260042)

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