Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TFPI antibody [EPR22977-16] - BSA and Azide free (ab264567)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22977-16] to TFPI - BSA and Azide free
  • Suitable for: IP, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-TFPI antibody [EPR22977-16] - BSA and Azide free
    See all TFPI primary antibodies

  • Description

    Rabbit monoclonal [EPR22977-16] to TFPI - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, Flow Cyt, WBmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human placenta lysate. HepG2, Caco-2, HUVEC and HT-29 lysates. Flow Cyt: HepG2 cells. IP: Human placenta lysate.

  • General notes

    ab264567 is the carrier-free version of ab260041. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22977-16

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-TFPI antibody [EPR22977-16] - BSA and Azide free (ab264567)
    Immunoprecipitation - Anti-TFPI antibody [EPR22977-16] - BSA and Azide free (ab264567)

    TFPI was immunoprecipitated from 0.35 mg human placenta lysate 10µg with ab260041 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260041 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Human placenta lysate 10µg

    Lane 2: ab260041 IP in human placenta lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab260041 in human placenta lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260041).

  • Flow Cytometry - Anti-TFPI antibody [EPR22977-16] - BSA and Azide free (ab264567)
    Flow Cytometry - Anti-TFPI antibody [EPR22977-16] - BSA and Azide free (ab264567)

    Flow cytometric analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling TFPI with ab260041 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260041).

  • Anti-TFPI antibody [EPR22977-16] - BSA and Azide free (ab264567)
    Anti-TFPI antibody [EPR22977-16] - BSA and Azide free (ab264567)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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