Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD31 antibody [EPR17260-265] - BSA and Azide free (ab232227)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17260-265] to CD31 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD31 antibody [EPR17260-265] - BSA and Azide free
    See all CD31 primary antibodies

  • Description

    Rabbit monoclonal [EPR17260-265] to CD31 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: bEnd.3 cell lysate; mouse platelet and lung lysates.

  • General notes

    Ab232227 is the carrier-free version of ab182982. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232227 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Immunoprecipitation - Anti-CD31 antibody [EPR17260-265] - BSA and Azide free (ab232227)
    Immunoprecipitation - Anti-CD31 antibody [EPR17260-265] - BSA and Azide free (ab232227)

    CD31 was immunoprecipitated from 1 mg bEnd.3 (mouse brain endothelioma cell line) whole cell lysate with ab182982 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab182982 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: bEnd.3 whole cell lysate  10 μg (Input).
    Lane 2: ab182982 IP in bEnd.3 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab182982 in bEnd.3 whole cell lysate (-)

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182982).

  • Western blot - Anti-CD31 antibody [EPR17260-265] - BSA and Azide free (ab232227)
    Western blot - Anti-CD31 antibody [EPR17260-265] - BSA and Azide free (ab232227)
    Lanes 1-2 : Anti-CD31 antibody [EPR17260-265] (ab182982) at 1/1000 dilution
    Lane 3 : Anti-CD31 antibody [EPR17260-265] (ab182982) at 1/2000 dilution

    Lane 1 : Mouse platelet lysate
    Lane 2 : Mouse lung lysate
    Lane 3 : bEnd.3 (mouse brain endothelioma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 82 kDa
    Observed band size: 110-130 kDa
    why is the actual band size different from the predicted?



    Blocking: 5% NFDM /TBST.

    Exposure time : Lane 1: 3 minutes; Lanes 2-3: 1 second.

    The expression MW observed is consistent with what has been described in the literature (PMID: 18388311; 12433657).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182982).

  • Anti-CD31 antibody [EPR17260-265] - BSA and Azide free (ab232227)
    Anti-CD31 antibody [EPR17260-265] - BSA and Azide free (ab232227)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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