Antibodies, Proteins, Kits and Reagents for Life Science

348 441 ₸ Product size

100 µl 348 441 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP3095] to CD31
Suitable for: IHC-P, WB, Flow Cyt
Reacts with: Human

Product name

Anti-CD31 antibody [EP3095]
See all CD31 primary antibodies
Description

Rabbit monoclonal [EP3095] to CD31
Host species

Rabbit
Specificity

This antibody gave positive results in mouse and rat tissue staining via IHC-P, but we have had difficulties obtaining consistent positive results in WB with mouse and rat samples. Thus, at this time we unfortunately cannot guarantee this antibody to recognize mouse and rat CD31, and optimization may be necessary when working with these species.

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human CD31 aa 700-800. The exact sequence is proprietary.
Positive control
- Human tonsil and uterus tissues; THP1 and Jurkat whole cell lysate (ab7899).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP3095
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095] (ab134168)

Immunohistochemical analysis of paraffin-embedded Human uterus tissue, staining CD31 using ab134168 at a 1/250 dilution. Note membrane staining of endothelial cells.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-CD31 antibody [EP3095] (ab134168)

Overlay histogram showing Jurkat cells stained with ab134168 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134168, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Western blot - Anti-CD31 antibody [EP3095] (ab134168)

All lanes : Anti-CD31 antibody [EP3095] (ab134168) at 1/1000 dilution

Lane 1 : THP1 cell lysate
Lane 2 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 82 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095] (ab134168)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, staining CD31 using ab134168 at a 1/250 dilution. Note membrane staining of endothelial cells.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095] (ab134168)

Immunohistochemical analysis of paraffin embedded normal Human spleen vessels tissue using ab134168 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095] (ab134168)

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma vessels tissue using ab134168 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095] (ab134168)

Immunohistochemical analysis of paraffin embedded Human Clear cell carcinoma of kidney tissue using ab134168 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095] (ab134168)

Immunohistochemical analysis of paraffin embedded normal Human lung tissue using ab134168 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-CD31 antibody [EP3095] (ab134168)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com