Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD31 antibody [EPR17260-254] (ab213175)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17260-254] to CD31
  • Suitable for: WB, IP, Sandwich ELISA
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-CD31 antibody [EPR17260-254]
    See all CD31 primary antibodies

  • Description

    Rabbit monoclonal [EPR17260-254] to CD31

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    sELISA
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse CD31 active protein; mouse and rat platelet and lung lysates; bEnd.3 whole cell lysate. IP: bEnd.3 whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Images

  • Sandwich ELISA - Anti-CD31 antibody [EPR17260-254] (ab213175)
    Sandwich ELISA - Anti-CD31 antibody [EPR17260-254] (ab213175)

    Example of mouse CD31 standard curve in 1X Cell Extraction Buffer PTR using recombinant CD31 antibody (ab213175) and a recombinant monoclonal antibody. Background-subtracted data values (mean +/- SD) are graphed.

    For a matched antibody pair set, see Mouse CD31 Matched Antibody Pair Kit (ab212065).

    For a complete ELISA kit, see Mouse CD31 ELISA kit (ab204527).

  • Western blot - Anti-CD31 antibody [EPR17260-254] (ab213175)
    Western blot - Anti-CD31 antibody [EPR17260-254] (ab213175)
    Lane 1 : Anti-CD31 antibody [EPR17260-254] (ab213175) at 1/20000 dilution
    Lane 2 : Anti-CD31 antibody [EPR17260-254] (ab213175) at 1/100000 dilution

    All lanes : Mouse CD31 active protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 81 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-CD31 antibody [EPR17260-254] (ab213175)
    Western blot - Anti-CD31 antibody [EPR17260-254] (ab213175)
    Lanes 1-4 : Anti-CD31 antibody [EPR17260-254] (ab213175) at 1/1000 dilution
    Lane 5 : Anti-CD31 antibody [EPR17260-254] (ab213175) at 1/2000 dilution

    Lane 1 : Mouse platelet lysate
    Lane 2 : Rat platelet lysate
    Lane 3 : Rat lung lysate
    Lane 4 : Mouse lung lysate
    Lane 5 : bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 81 kDa
    Observed band size: 110-130 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-3: 10 seconds; Lane 4/5: 1 second.

    The expression MW observed is consistent with what has been described in the literature (PMID: 18388311; 12433657).

  • Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] (ab213175)
    Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] (ab213175)

    CD31 was immunoprecipitated from 1 mg of bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate with ab213175 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab213175 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1: bEnd.3 whole cell lysate 10µg (Input). Lane 2: ab213175 IP in bEnd.3 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213175 in bEnd.3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Anti-CD31 antibody [EPR17260-254] (ab213175)
    Anti-CD31 antibody [EPR17260-254] (ab213175)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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