Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Wednesday March 10, 2021

Anti-CD31 antibody [EPR17260-254] (ab213175)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17260-254] to CD31
Suitable for: WB, IP, Sandwich ELISA
Reacts with: Mouse, Rat

Product name

Anti-CD31 antibody [EPR17260-254]
See all CD31 primary antibodies
Description

Rabbit monoclonal [EPR17260-254] to CD31
Host species

Rabbit

Tested Applications & Species

Application	Species
IP	Mouse
sELISA	Mouse
WB	Mouse Rat

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse CD31 active protein; mouse and rat platelet and lung lysates; bEnd.3 whole cell lysate. IP: bEnd.3 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17260-254
Isotype

IgG
Research areas

Sandwich ELISA - Anti-CD31 antibody [EPR17260-254] (ab213175)

Example of mouse CD31 standard curve in 1X Cell Extraction Buffer PTR using recombinant CD31 antibody (ab213175) and a recombinant monoclonal antibody. Background-subtracted data values (mean +/- SD) are graphed.

For a matched antibody pair set, see Mouse CD31 Matched Antibody Pair Kit (ab212065).

For a complete ELISA kit, see Mouse CD31 ELISA kit (ab204527).
Western blot - Anti-CD31 antibody [EPR17260-254] (ab213175)

Lane 1 : Anti-CD31 antibody [EPR17260-254] (ab213175) at 1/20000 dilution
Lane 2 : Anti-CD31 antibody [EPR17260-254] (ab213175) at 1/100000 dilution

All lanes : Mouse CD31 active protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 81 kDa
Observed band size: 100 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-CD31 antibody [EPR17260-254] (ab213175)

Lanes 1-4 : Anti-CD31 antibody [EPR17260-254] (ab213175) at 1/1000 dilution
Lane 5 : Anti-CD31 antibody [EPR17260-254] (ab213175) at 1/2000 dilution

Lane 1 : Mouse platelet lysate
Lane 2 : Rat platelet lysate
Lane 3 : Rat lung lysate
Lane 4 : Mouse lung lysate
Lane 5 : bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 81 kDa
Observed band size: 110-130 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1-3: 10 seconds; Lane 4/5: 1 second.

The expression MW observed is consistent with what has been described in the literature (PMID: 18388311; 12433657).
Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] (ab213175)

CD31 was immunoprecipitated from 1 mg of bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate with ab213175 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab213175 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

Lane 1: bEnd.3 whole cell lysate 10µg (Input). Lane 2: ab213175 IP in bEnd.3 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213175 in bEnd.3 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds.
Anti-CD31 antibody [EPR17260-254] (ab213175)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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