Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Gli3 antibody [EPR4594] (ab181130)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4594] to Gli3
  • Suitable for: ICC/IF, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Gli3 antibody [EPR4594]
    See all Gli3 primary antibodies

  • Description

    Rabbit monoclonal [EPR4594] to Gli3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 293 lysate ICC/IF: 293 cells and human kidney tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (ab181130)
    Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (ab181130) This image is courtesy of an abreview by Lindsey Fitzsimons

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue labelling Gli3 (green) with ab181130 at 1/100 dilution. Antigen retrieval was performed using Tris/EDTA buffer pH 9 for 30 minutes, a HRP-conjugated goat anti-rabbit IgG (H+L) (Alexa Fluor® 488) ab150078 was used as the secondary antibody (1/1000). Sections were co-stained with Monoclonal Anti-tubulin (1/1000). Magnification at x100.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (ab181130)
    Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (ab181130)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue labelling Gli3 (green) with ab181130 at 1/100 dilution. Antigen retrieval was performed using Tris/EDTA buffer pH 9 for 30 minutes, a HRP-conjugated goat anti-rabbit IgG (H+L) (Alexa Fluor® 488) ab150078 was used as the secondary antibody (1/1000). Sections were co-stained with Monoclonal Anti-tubulin (1/1000). Magnification at x40.

    See Abreview

  • Western blot - Anti-Gli3 antibody [EPR4594] (ab181130)
    Western blot - Anti-Gli3 antibody [EPR4594] (ab181130)
    Anti-Gli3 antibody [EPR4594] (ab181130) at 1/1000 dilution + 293 lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 190 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (ab181130)
    Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (ab181130)

    Immunofluorescence analysis of 293 cells (fixative 4% paraformaldehyde) labeling Gli3 with ab181130 at a 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor 488™) secondary used at a 1/200 diution.

  • Anti-Gli3 antibody [EPR4594] (ab181130)
    Anti-Gli3 antibody [EPR4594] (ab181130)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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