Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4594] to Gli3
Suitable for: ICC/IF, WB
Reacts with: Human

Product name

Anti-Gli3 antibody [EPR4594]
See all Gli3 primary antibodies
Description

Rabbit monoclonal [EPR4594] to Gli3
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: 293 lysate ICC/IF: 293 cells and human kidney tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4594
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (ab181130) This image is courtesy of an abreview by Lindsey Fitzsimons

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue labelling Gli3 (green) with ab181130 at 1/100 dilution. Antigen retrieval was performed using Tris/EDTA buffer pH 9 for 30 minutes, a HRP-conjugated goat anti-rabbit IgG (H+L) (Alexa Fluor® 488) ab150078 was used as the secondary antibody (1/1000). Sections were co-stained with Monoclonal Anti-tubulin (1/1000). Magnification at x100.
See Abreview
Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (ab181130)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue labelling Gli3 (green) with ab181130 at 1/100 dilution. Antigen retrieval was performed using Tris/EDTA buffer pH 9 for 30 minutes, a HRP-conjugated goat anti-rabbit IgG (H+L) (Alexa Fluor® 488) ab150078 was used as the secondary antibody (1/1000). Sections were co-stained with Monoclonal Anti-tubulin (1/1000). Magnification at x40.
See Abreview
Western blot - Anti-Gli3 antibody [EPR4594] (ab181130)

Anti-Gli3 antibody [EPR4594] (ab181130) at 1/1000 dilution + 293 lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 190 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (ab181130)

Immunofluorescence analysis of 293 cells (fixative 4% paraformaldehyde) labeling Gli3 with ab181130 at a 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor 488™) secondary used at a 1/200 diution.
Anti-Gli3 antibody [EPR4594] (ab181130)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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