Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 02, 2021

Anti-FACL4 antibody [EPR8640] (ab155282)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8640] to FACL4
Suitable for: IHC-P, IP, WB, Flow Cyt, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-FACL4 antibody [EPR8640]
See all FACL4 primary antibodies
Description

Rabbit monoclonal [EPR8640] to FACL4
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human FACL4. The exact sequence is proprietary.
Positive control
- WB: HepG2, HeLa and 293T cell lysates and human fetal kidney tissue, mouse brain tissue, rat brain, kidney and spleen tissue lysates. IHC-P: human hepatocellular carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt: Permeabilized 293T cells. IP: HepG2 whole cell lysate (ab7900).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR8640
Isotype

IgG
Research areas

Western blot - Anti-FACL4 antibody [EPR8640] (ab155282)

All lanes : Anti-FACL4 antibody [EPR8640] (ab155282) at 1/1000 dilution (purified)

Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 79 kDa
Observed band size: 79 kDa

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-FACL4 antibody [EPR8640] (ab155282)

Anti-FACL4 antibody [EPR8640] (ab155282) at 1/50000 dilution (purified) + HepG2 whole cell lysate at 20 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 79 kDa
Observed band size: 79 kDa

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-FACL4 antibody [EPR8640] (ab155282)

All lanes : Anti-FACL4 antibody [EPR8640] (ab155282) at 1/10000 dilution (purified)

Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293 whole cell lysate
Lane 3 : Human fetal kidney tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-conjugate goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 79 kDa
Observed band size: 79 kDa

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FACL4 antibody [EPR8640] (ab155282)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling FACL4 with purified ab155282 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-FACL4 antibody [EPR8640] (ab155282)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling FACL4 with purified ab155282 at 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/200) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000).
Flow Cytometry - Anti-FACL4 antibody [EPR8640] (ab155282)

Flow Cytometry analysis of 293T cells labelling FACL4 with purified ab155282 at 1/100 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Immunoprecipitation - Anti-FACL4 antibody [EPR8640] (ab155282)

ab155282 (purified) at 1/30 immunoprecipitating FACL4 in HepG2 whole cell lysate.
Lane 1 (input): HepG2 whole cell lysate (10µg)
Lane 2 (+): ab155282 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab155282 in HepG2 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-FACL4 antibody [EPR8640] (ab155282)

All lanes : Anti-FACL4 antibody [EPR8640] (ab155282) at 1/10000 dilution (unpurified)

Lane 1 : HepG2 lysate
Lane 2 : HeLa lysate
Lane 3 : 293T lysate
Lane 4 : Fetal kidney lysate, human

Lysates/proteins at 10 µg per lane.

Predicted band size: 79 kDa
Western blot - Anti-FACL4 antibody [EPR8640] (ab155282)

All lanes : Anti-FACL4 antibody [EPR8640] (ab155282) at 1/10000 dilution (unpurified)

Lane 1 : Rat brain tissue lysate
Lane 2 : Rat heart tissue lysate
Lane 3 : Rat kidney tissue lysate
Lane 4 : Rat spleen tissue lysate

Predicted band size: 79 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute
Flow Cytometry - Anti-FACL4 antibody [EPR8640] (ab155282)

Flow cytometric analysis of permeabilized 293T cells labelling FACL4 with unpurified ab155282 at a dilution of 1/10 (red) compared to a rabbit IgG (negative) (green).
Anti-FACL4 antibody [EPR8640] (ab155282)

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